Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 512490..513126 Replicon chromosome
Accession NZ_CP126083
Organism Bacillus subtilis strain C1_9

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QNK02_RS02620 Protein ID WP_003156187.1
Coordinates 512776..513126 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag QNK02_RS02615 Protein ID WP_003225183.1
Coordinates 512490..512771 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QNK02_RS02595 (QNK02_02595) 508850..509449 - 600 WP_041850724.1 rhomboid family intramembrane serine protease -
QNK02_RS02600 (QNK02_02600) 509544..509909 + 366 WP_015252768.1 holo-ACP synthase -
QNK02_RS02605 (QNK02_02605) 510075..511091 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
QNK02_RS02610 (QNK02_02610) 511205..512374 + 1170 WP_015252766.1 alanine racemase -
QNK02_RS02615 (QNK02_02615) 512490..512771 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QNK02_RS02620 (QNK02_02620) 512776..513126 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QNK02_RS02625 (QNK02_02625) 513241..514065 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
QNK02_RS02630 (QNK02_02630) 514070..514435 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
QNK02_RS02635 (QNK02_02635) 514439..514840 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
QNK02_RS02640 (QNK02_02640) 514852..515859 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
QNK02_RS02645 (QNK02_02645) 515921..516250 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
QNK02_RS02650 (QNK02_02650) 516247..516729 + 483 WP_003234299.1 anti-sigma B factor RsbW -
QNK02_RS02655 (QNK02_02655) 516695..517483 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
QNK02_RS02660 (QNK02_02660) 517483..518082 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T282590 WP_003156187.1 NZ_CP126083:512776-513126 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT282590 WP_003225183.1 NZ_CP126083:512490-512771 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References