Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 531131..531767 Replicon chromosome
Accession NZ_CP126081
Organism Bacillus pumilus strain B2_10

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag QNH35_RS02560 Protein ID WP_003214169.1
Coordinates 531417..531767 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag QNH35_RS02555 Protein ID WP_003214273.1
Coordinates 531131..531412 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QNH35_RS02535 (QNH35_02535) 527277..527882 - 606 WP_126741642.1 rhomboid family intramembrane serine protease -
QNH35_RS02540 (QNH35_02540) 527977..528342 + 366 WP_217013202.1 holo-ACP synthase -
QNH35_RS02545 (QNH35_02545) 528503..529519 + 1017 WP_113754968.1 outer membrane lipoprotein carrier protein LolA -
QNH35_RS02550 (QNH35_02550) 529655..530836 + 1182 WP_217013203.1 alanine racemase -
QNH35_RS02555 (QNH35_02555) 531131..531412 + 282 WP_003214273.1 hypothetical protein Antitoxin
QNH35_RS02560 (QNH35_02560) 531417..531767 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QNH35_RS02565 (QNH35_02565) 531881..532711 + 831 WP_044140569.1 RsbT co-antagonist protein RsbRA -
QNH35_RS02570 (QNH35_02570) 532716..533084 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
QNH35_RS02575 (QNH35_02575) 533087..533488 + 402 WP_283924168.1 anti-sigma regulatory factor -
QNH35_RS02580 (QNH35_02580) 533499..534506 + 1008 WP_065097543.1 PP2C family protein-serine/threonine phosphatase -
QNH35_RS02585 (QNH35_02585) 534566..534895 + 330 WP_044140572.1 anti-sigma factor antagonist -
QNH35_RS02590 (QNH35_02590) 534892..535380 + 489 WP_044140573.1 anti-sigma B factor RsbW -
QNH35_RS02595 (QNH35_02595) 535346..536134 + 789 WP_012009000.1 RNA polymerase sigma factor SigB -
QNH35_RS02600 (QNH35_02600) 536134..536733 + 600 WP_044140574.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T282587 WP_003214169.1 NZ_CP126081:531417-531767 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT282587 WP_003214273.1 NZ_CP126081:531131-531412 [Bacillus pumilus]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References