Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 541149..541802 Replicon chromosome
Accession NZ_CP126078
Organism Brevibacillus agri strain ACCC03016

Toxin (Protein)


Gene name mazF Uniprot ID J2PLP3
Locus tag QNK09_RS02870 Protein ID WP_005830205.1
Coordinates 541452..541802 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3M8AYL7
Locus tag QNK09_RS02865 Protein ID WP_025845698.1
Coordinates 541149..541448 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QNK09_RS02845 (QNK09_02845) 536627..537664 + 1038 WP_005830195.1 DUF4367 domain-containing protein -
QNK09_RS02850 (QNK09_02850) 537871..538992 + 1122 WP_283858603.1 hypothetical protein -
QNK09_RS02855 (QNK09_02855) 539325..539765 + 441 WP_005830198.1 hypothetical protein -
QNK09_RS02860 (QNK09_02860) 539768..540970 + 1203 WP_007776941.1 alanine racemase -
QNK09_RS02865 (QNK09_02865) 541149..541448 + 300 WP_025845698.1 CopG family ribbon-helix-helix protein Antitoxin
QNK09_RS02870 (QNK09_02870) 541452..541802 + 351 WP_005830205.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
QNK09_RS02875 (QNK09_02875) 542104..542460 + 357 WP_007776946.1 anti-sigma regulatory factor -
QNK09_RS02880 (QNK09_02880) 542454..543455 + 1002 WP_007776949.1 PP2C family protein-serine/threonine phosphatase -
QNK09_RS02885 (QNK09_02885) 543481..543807 + 327 WP_005830210.1 anti-sigma factor antagonist -
QNK09_RS02890 (QNK09_02890) 543807..544301 + 495 WP_005830211.1 anti-sigma B factor RsbW -
QNK09_RS02895 (QNK09_02895) 544270..545067 + 798 WP_005830213.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-52)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12917.86 Da        Isoelectric Point: 4.8435

>T282584 WP_005830205.1 NZ_CP126078:541452-541802 [Brevibacillus agri]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVNESLQISLGLIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11323.26 Da        Isoelectric Point: 10.3594

>AT282584 WP_025845698.1 NZ_CP126078:541149-541448 [Brevibacillus agri]
MVGGGFVLSKSNTKRIMISLPQHLLQEVDGVIQKEKSNRSEFIRQAMHLYLKERKKRTIRETMQKGYMEMAKINLKMASE
AFGAEEEADHTLVRLVSGV

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J2PLP3

Antitoxin

Source ID Structure
AlphaFold DB A0A3M8AYL7

References