Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 3188448..3188880 Replicon chromosome
Accession NZ_CP126076
Organism Clostridioides difficile strain CDI-01

Toxin (Protein)


Gene name zeta Uniprot ID R4ZD79
Locus tag ITJ31_RS14610 Protein ID WP_003423072.1
Coordinates 3188448..3188606 (-) Length 53 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag ITJ31_RS14615 Protein ID WP_000301765.1
Coordinates 3188608..3188880 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ITJ31_RS14575 (ITJ31_014575) 3184010..3184210 - 201 WP_021405844.1 helix-turn-helix domain-containing protein -
ITJ31_RS14580 (ITJ31_014580) 3184662..3184910 - 249 WP_002323370.1 helix-turn-helix domain-containing protein -
ITJ31_RS14585 (ITJ31_014585) 3184917..3185333 - 417 WP_003423075.1 sigma factor-like helix-turn-helix DNA-binding protein -
ITJ31_RS14590 (ITJ31_014590) 3185781..3186659 - 879 WP_003423074.1 ParA family protein -
ITJ31_RS14595 (ITJ31_014595) 3186762..3187022 - 261 Protein_2799 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
ITJ31_RS14600 (ITJ31_014600) 3187192..3187930 - 739 Protein_2800 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
ITJ31_RS14605 (ITJ31_014605) 3188040..3188396 - 357 Protein_2801 AAA family ATPase -
ITJ31_RS14610 (ITJ31_014610) 3188448..3188606 - 159 WP_003423072.1 zeta toxin family protein Toxin
ITJ31_RS14615 (ITJ31_014615) 3188608..3188880 - 273 WP_000301765.1 antitoxin Antitoxin
ITJ31_RS14620 (ITJ31_014620) 3188897..3189112 - 216 WP_002321610.1 peptide-binding protein -
ITJ31_RS14625 (ITJ31_014625) 3189299..3189658 + 360 WP_003423065.1 helix-turn-helix transcriptional regulator -
ITJ31_RS14630 (ITJ31_014630) 3189766..3189954 - 189 WP_009269672.1 cysteine-rich KTR domain-containing protein -
ITJ31_RS14635 (ITJ31_014635) 3190159..3190350 - 192 WP_003423063.1 hypothetical protein -
ITJ31_RS14640 (ITJ31_014640) 3190363..3191007 - 645 WP_003423062.1 ABC-2 transporter permease -
ITJ31_RS14645 (ITJ31_014645) 3191004..3191867 - 864 WP_003423060.1 ABC transporter ATP-binding protein -
ITJ31_RS14650 (ITJ31_014650) 3191868..3192245 - 378 WP_003423058.1 GntR family transcriptional regulator -
ITJ31_RS14655 (ITJ31_014655) 3192366..3193277 - 912 WP_003423057.1 conjugal transfer protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element erm(B) - 3182546..3209903 27357


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(26-50)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 53 a.a.        Molecular weight: 5776.53 Da        Isoelectric Point: 10.3088

>T282582 WP_003423072.1 NZ_CP126076:c3188606-3188448 [Clostridioides difficile]
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRRR

Download         Length: 159 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT282582 WP_000301765.1 NZ_CP126076:c3188880-3188608 [Clostridioides difficile]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R4ZD79

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References