Detailed information of TA system TA028254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2119376..2119890
• Replicon: chromosome
• Accession: NC_018608
• Organism: Staphylococcus aureus 08BA02176

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: C248_RS10515
• Protein ID: WP_000581792.1
• Coordinates: 2119376..2119585 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: C248_RS10520
• Protein ID: WP_000138932.1
• Coordinates: 2119597..2119890 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C248_RS10495	2114887..2116242	-	1356	WP_000611457.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
C248_RS10500	2116257..2117327	-	1071	WP_000159641.1	D-alanine--D-alanine ligase	-
C248_RS10505	2117645..2118847	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
C248_RS10510	2119111..2119248	-	138	WP_000828354.1	hypothetical protein	-
C248_RS10515	2119376..2119585	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
C248_RS10520	2119597..2119890	-	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
C248_RS10525	2120056..2121540	+	1485	WP_000571550.1	cardiolipin synthase	-
C248_RS10530	2121568..2122215	+	648	WP_001187614.1	HD domain-containing protein	-
C248_RS10540	2122495..2122815	+	321	WP_001825748.1	hypothetical protein	-
C248_RS10545	2122812..2124128	-	1317	WP_001066880.1	ISL3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2122812..2124128	1316

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T28254 WP_000581792.1 NC_018608:c2119585-2119376 [Staphylococcus aureus 08BA02176]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T28254 NC_018608:c2119585-2119376 [Staphylococcus aureus 08BA02176]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT28254 WP_000138932.1 NC_018608:c2119890-2119597 [Staphylococcus aureus 08BA02176]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT28254 NC_018608:c2119890-2119597 [Staphylococcus aureus 08BA02176]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13