Detailed information of TA system TA028253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2099640..2100169
• Replicon: chromosome
• Accession: NC_018608
• Organism: Staphylococcus aureus 08BA02176

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C248_RS10425
• Protein ID: WP_000621175.1
• Coordinates: 2099640..2100002 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: C248_RS10430
• Protein ID: WP_000948331.1
• Coordinates: 2099999..2100169 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C248_RS10400	2096619..2097389	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
C248_RS10405	2097364..2097843	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
C248_RS10410	2097845..2098171	-	327	WP_001052491.1	anti-sigma factor antagonist	-
C248_RS10415	2098290..2099291	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
C248_RS10425	2099640..2100002	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C248_RS10430	2099999..2100169	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
C248_RS10435	2100254..2101402	-	1149	WP_001281139.1	alanine racemase	-
C248_RS10440	2101468..2101827	-	360	WP_000581197.1	holo-ACP synthase	-
C248_RS10445	2101831..2102322	-	492	WP_001286980.1	PH domain-containing protein	-
C248_RS10450	2102315..2103892	-	1578	WP_001294650.1	PH domain-containing protein	-
C248_RS10455	2103885..2104364	-	480	WP_001287081.1	hypothetical protein	-
C248_RS10460	2104573..2105132	-	560	Protein_1974	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T28253 WP_000621175.1 NC_018608:c2100002-2099640 [Staphylococcus aureus 08BA02176]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T28253 NC_018608:c2100002-2099640 [Staphylococcus aureus 08BA02176]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT28253 WP_000948331.1 NC_018608:c2100169-2099999 [Staphylococcus aureus 08BA02176]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT28253 NC_018608:c2100169-2099999 [Staphylococcus aureus 08BA02176]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1