Detailed information of TA system TA028242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 470310..470978
• Replicon: chromosome
• Accession: NC_018594
• Organism: Streptococcus pneumoniae SPNA45

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: I0T4C6
• Locus tag: SPNA45_RS02780
• Protein ID: WP_001132287.1
• Coordinates: 470310..470489 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: G0IC64
• Locus tag: SPNA45_RS02785
• Protein ID: WP_000961810.1
• Coordinates: 470526..470978 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPNA45_RS02740	465371..466414	+	1044	WP_001031657.1	hypothetical protein	-
SPNA45_RS02745	466430..466612	+	183	WP_000655516.1	DUF3173 domain-containing protein	-
SPNA45_RS02750	466614..466877	+	264	WP_041852767.1	Arm DNA-binding domain-containing protein	-
SPNA45_RS02755	467027..467740	+	714	WP_014931841.1	site-specific integrase	-
SPNA45_RS02765	468160..469431	-	1272	WP_001113213.1	replication-associated recombination protein A	-
SPNA45_RS02770	469720..469935	+	216	WP_075329187.1	hypothetical protein	-
SPNA45_RS02775	469925..470173	+	249	WP_000797240.1	hypothetical protein	-
SPNA45_RS02780	470310..470489	+	180	WP_001132287.1	type II toxin-antitoxin system HicA family toxin	Toxin
SPNA45_RS02785	470526..470978	+	453	WP_000961810.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
SPNA45_RS02790	471269..471739	+	471	WP_000257093.1	DUF3013 family protein	-
SPNA45_RS02795	471795..472337	+	543	WP_000891118.1	hypothetical protein	-
SPNA45_RS02800	472387..472821	+	435	WP_000421871.1	NUDIX hydrolase	-
SPNA45_RS02805	472818..473291	+	474	WP_000631890.1	GNAT family N-acetyltransferase	-
SPNA45_RS02810	473429..474379	+	951	WP_000451159.1	50S ribosomal protein L11 methyltransferase	-
SPNA45_RS02815	474381..475124	+	744	WP_001188201.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (17-114)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6697.91 Da
• Isoelectric Point: 11.0174

>T28242 WP_001132287.1 NC_018594:470310-470489 [Streptococcus pneumoniae SPNA45]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITIPHGELNKYTERGIRKQAGL

Nucleotide

• Length: 180 bp

>T28242 NC_018594:470310-470489 [Streptococcus pneumoniae SPNA45]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGTTCCCA
TATTAAAATGGAGAAGCAAGGGGAAAGACCTATCACAATCCCTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16659.46 Da
• Isoelectric Point: 3.7220

>AT28242 WP_000961810.1 NC_018594:470526-470978 [Streptococcus pneumoniae SPNA45]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Nucleotide

• Length: 453 bp

>AT28242 NC_018594:470526-470978 [Streptococcus pneumoniae SPNA45]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Structures

Toxin

• AlphaFold DB: I0T4C6

Antitoxin

• PDB: 5YRZ
• AlphaFold DB: G0IC64