Detailed information of TA system TA028239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 170704..171335
• Replicon: plasmid pGKT2
• Accession: NC_018580
• Organism: Gordonia sp. KTR9

Toxin (Protein)

• Gene name: doc
• Uniprot ID: E1R0T1
• Locus tag: KTR9_RS00725
• Protein ID: WP_014924775.1
• Coordinates: 170704..171141 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: E1R0C5
• Locus tag: KTR9_RS00730
• Protein ID: WP_014924776.1
• Coordinates: 171138..171335 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTR9_RS00705	166345..166563	-	219	WP_044505541.1	hypothetical protein	-
KTR9_RS00720	170139..170369	-	231	WP_014924774.1	helix-turn-helix domain-containing protein	-
KTR9_RS00725	170704..171141	-	438	WP_014924775.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KTR9_RS00730	171138..171335	-	198	WP_014924776.1	hypothetical protein	Antitoxin
KTR9_RS00735	171588..171857	-	270	WP_014924777.1	hypothetical protein	-
KTR9_RS00740	171996..172379	-	384	WP_014924778.1	hypothetical protein	-
KTR9_RS00745	172590..173333	-	744	WP_148281130.1	hypothetical protein	-
KTR9_RS00750	173354..173818	-	465	WP_014924780.1	hypothetical protein	-
KTR9_RS00755	173862..175049	-	1188	WP_083888920.1	hypothetical protein	-
KTR9_RS27580	175226..175390	-	165	WP_158409740.1	hypothetical protein	-
KTR9_RS00760	175387..175716	-	330	WP_014924782.1	hypothetical protein	-
KTR9_RS27585	175769..175933	-	165	WP_014924783.1	hypothetical protein	-
KTR9_RS00765	175953..176318	-	366	WP_014924784.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..182454	182454

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (24-124)

Antitoxin

• Antitoxin ParD (1-48)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 16043.10 Da
• Isoelectric Point: 4.7677

>T28239 WP_014924775.1 NC_018580:c171141-170704 [Gordonia sp. KTR9]
MTDFLDREDVLTAGSVACGEQLIVRDEGLLQAAVARPLTSVFGVEAYPNLWDKAAALMHSLARNHPFVDGNKRTSWASAN
VFLHINGIMPTVDLDVDRAEVFVNDVAKGVIDEWADIADGLHYLYGLSHRSLPINSPVKDDRRER

Nucleotide

• Length: 438 bp

>T28239 NC_018580:c171141-170704 [Gordonia sp. KTR9]
ATGACAGACTTTCTGGATCGAGAGGACGTCCTCACGGCCGGTTCGGTGGCGTGTGGCGAGCAGCTGATCGTGCGGGACGA
GGGTCTACTCCAGGCCGCCGTCGCCAGACCGCTGACCTCAGTGTTCGGTGTTGAGGCGTACCCGAACCTCTGGGACAAAG
CAGCGGCGCTGATGCACTCGCTCGCGCGCAATCACCCGTTCGTCGACGGCAACAAGCGCACCTCCTGGGCGAGTGCCAAC
GTCTTCCTACACATCAACGGCATCATGCCGACTGTGGATCTTGACGTCGATCGCGCCGAGGTGTTCGTCAACGACGTCGC
CAAAGGTGTCATCGACGAGTGGGCCGACATCGCGGACGGCCTGCACTACCTGTACGGACTGAGCCATCGATCGCTGCCCA
TCAACTCGCCCGTTAAGGATGATCGCCGTGAACGATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7216.03 Da
• Isoelectric Point: 6.2169

>AT28239 WP_014924776.1 NC_018580:c171335-171138 [Gordonia sp. KTR9]
MPSLNIEFTEEEHAQIKAAAESAGVSLKPFVHDAAVDRASEHRRRVLDAAKSVASWSSELNERLR

Nucleotide

• Length: 198 bp

>AT28239 NC_018580:c171335-171138 [Gordonia sp. KTR9]
ATGCCGTCACTGAACATCGAGTTCACCGAGGAAGAGCACGCTCAGATCAAGGCCGCCGCTGAGAGCGCCGGCGTCTCGCT
CAAGCCGTTTGTCCACGACGCCGCGGTCGACCGGGCGAGCGAGCATCGTCGCCGGGTCCTCGACGCCGCGAAGTCGGTCG
CATCCTGGTCCTCGGAGCTGAACGAAAGGCTGCGATGA

Structures

Toxin

• AlphaFold DB: E1R0T1

Antitoxin

• AlphaFold DB: E1R0C5