Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3913294..3913930 Replicon chromosome
Accession NZ_CP125762
Organism Bacillus subtilis strain KRS015

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QLQ05_RS21000 Protein ID WP_003156187.1
Coordinates 3913294..3913644 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag QLQ05_RS21005 Protein ID WP_003225183.1
Coordinates 3913649..3913930 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QLQ05_RS20960 (3908338) 3908338..3908937 - 600 WP_003246608.1 phosphoserine phosphatase RsbX -
QLQ05_RS20965 (3908937) 3908937..3909725 - 789 WP_003246715.1 RNA polymerase sigma factor SigB -
QLQ05_RS20970 (3909691) 3909691..3910173 - 483 WP_003234299.1 anti-sigma B factor RsbW -
QLQ05_RS20975 (3910170) 3910170..3910499 - 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
QLQ05_RS20980 (3910561) 3910561..3911568 - 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
QLQ05_RS20985 (3911580) 3911580..3911981 - 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
QLQ05_RS20990 (3911985) 3911985..3912350 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
QLQ05_RS20995 (3912355) 3912355..3913179 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
QLQ05_RS21000 (3913294) 3913294..3913644 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QLQ05_RS21005 (3913649) 3913649..3913930 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QLQ05_RS21010 (3914046) 3914046..3915215 - 1170 WP_003234284.1 alanine racemase -
QLQ05_RS21015 (3915330) 3915330..3916346 - 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
QLQ05_RS21020 (3916512) 3916512..3916877 - 366 WP_003234281.1 holo-ACP synthase -
QLQ05_RS21025 (3916972) 3916972..3917571 + 600 WP_003246687.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T282385 WP_003156187.1 NZ_CP125762:c3913644-3913294 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT282385 WP_003225183.1 NZ_CP125762:c3913930-3913649 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References