Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4104684..4105260 Replicon chromosome
Accession NZ_CP125731
Organism Escherichia coli strain GYB01

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QMW19_RS19875 Protein ID WP_001297643.1
Coordinates 4104973..4105260 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag QMW19_RS19870 Protein ID WP_000063160.1
Coordinates 4104684..4104986 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMW19_RS19855 (4101280) 4101280..4103430 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QMW19_RS19860 (4103480) 4103480..4103683 + 204 WP_000467859.1 YbdD/YjiX family protein -
QMW19_RS19865 (4103694) 4103694..4104650 + 957 WP_001297640.1 GTPase -
QMW19_RS19870 (4104684) 4104684..4104986 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
QMW19_RS19875 (4104973) 4104973..4105260 - 288 WP_001297643.1 BrnT family toxin Toxin
QMW19_RS19880 (4105459) 4105459..4106155 - 697 Protein_3885 IS1 family transposase -
QMW19_RS19885 (4106470) 4106470..4107702 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
QMW19_RS19890 (4107743) 4107743..4109023 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4105459..4105668 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T282300 WP_001297643.1 NZ_CP125731:c4105260-4104973 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT282300 WP_000063160.1 NZ_CP125731:c4104986-4104684 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References