Detailed information of TA system TA282280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 919557..920086
• Replicon: chromosome
• Accession: NZ_CP125718
• Organism: Staphylococcus casei strain DSM 15096

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: QMO72_RS04275
• Protein ID: WP_069822705.1
• Coordinates: 919724..920086 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1S2MIN4
• Locus tag: QMO72_RS04270
• Protein ID: WP_046837773.1
• Coordinates: 919557..919727 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMO72_RS04245 (QMO72_04245)	915327..915806	+	480	WP_103266273.1	PH domain-containing protein	-
QMO72_RS04250 (QMO72_04250)	915799..917301	+	1503	WP_103266274.1	PH domain-containing protein	-
QMO72_RS04255 (QMO72_04255)	917294..917800	+	507	WP_103266275.1	PH domain-containing protein	-
QMO72_RS04260 (QMO72_04260)	917855..918205	+	351	WP_069822707.1	holo-ACP synthase	-
QMO72_RS04265 (QMO72_04265)	918321..919469	+	1149	WP_103266276.1	alanine racemase	-
QMO72_RS04270 (QMO72_04270)	919557..919727	+	171	WP_046837773.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
QMO72_RS04275 (QMO72_04275)	919724..920086	+	363	WP_069822705.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
QMO72_RS04280 (QMO72_04280)	920436..921437	+	1002	WP_103266277.1	PP2C family protein-serine/threonine phosphatase	-
QMO72_RS04285 (QMO72_04285)	921517..921843	+	327	WP_046837770.1	anti-sigma factor antagonist	-
QMO72_RS04290 (QMO72_04290)	921845..922327	+	483	WP_069822703.1	anti-sigma B factor RsbW	-
QMO72_RS04295 (QMO72_04295)	922302..923072	+	771	WP_063410319.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13490.59 Da
• Isoelectric Point: 10.0960

>T282280 WP_069822705.1 NZ_CP125718:919724-920086 [Staphylococcus casei]
MMRRGDVYLADLSPVQGSEQGGIRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSEDKMREVNNAIDISLGLHSVRTNKS

Nucleotide

• Length: 363 bp

>T282280 NZ_CP125718:919724-920086 [Staphylococcus casei]
ATGATGAGAAGAGGCGACGTTTATTTAGCTGATTTATCACCAGTGCAAGGGTCTGAACAAGGGGGAATTAGACCTGTCGT
GATTATACAAAACGATACTGGAAATAAATATAGTCCTACCGTAATTGTAGCAGCAATTACTGGTAGGATAAATAAAGCTA
AGATACCAACACATGTAGAAATAGAAAAAAGTAAGTATAAGTTAGATAAAGATTCTGTTATTTTGTTAGAACAAATTAGA
ACTTTAGATAAAAACAGACTGAAAGAAAAATTAACTTATCTCTCTGAAGATAAAATGAGAGAAGTAAACAATGCTATCGA
CATAAGCTTAGGATTACATAGTGTTAGGACAAATAAATCCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6320.97 Da
• Isoelectric Point: 4.0121

>AT282280 WP_046837773.1 NZ_CP125718:919557-919727 [Staphylococcus casei]
MVSFTQSRNHSIEQLLKEGYSQMADLNLSLAAEAFPFECEACDCNEVYISSKNNNE

Nucleotide

• Length: 171 bp

>AT282280 NZ_CP125718:919557-919727 [Staphylococcus casei]
ATGGTAAGTTTTACTCAAAGCAGAAATCACAGTATTGAACAATTACTAAAGGAAGGCTATTCTCAAATGGCCGACTTAAA
CCTCTCCCTTGCAGCAGAAGCATTTCCATTTGAATGTGAAGCTTGTGATTGCAACGAAGTCTACATCAGCTCTAAGAATA
ACAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1S2MIN4