Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518258..518894 Replicon chromosome
Accession NZ_CP125660
Organism Bacillus subtilis strain GUCC48

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QLH41_RS02640 Protein ID WP_003156187.1
Coordinates 518544..518894 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag QLH41_RS02635 Protein ID WP_003225183.1
Coordinates 518258..518539 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QLH41_RS02615 (514617) 514617..515216 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
QLH41_RS02620 (515311) 515311..515676 + 366 WP_003234281.1 holo-ACP synthase -
QLH41_RS02625 (515842) 515842..516858 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
QLH41_RS02630 (516973) 516973..518142 + 1170 WP_003234284.1 alanine racemase -
QLH41_RS02635 (518258) 518258..518539 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QLH41_RS02640 (518544) 518544..518894 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QLH41_RS02645 (519009) 519009..519833 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
QLH41_RS02650 (519838) 519838..520203 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
QLH41_RS02655 (520207) 520207..520608 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
QLH41_RS02660 (520620) 520620..521627 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
QLH41_RS02665 (521689) 521689..522018 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
QLH41_RS02670 (522015) 522015..522497 + 483 WP_003234299.1 anti-sigma B factor RsbW -
QLH41_RS02675 (522463) 522463..523251 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
QLH41_RS02680 (523251) 523251..523850 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T282249 WP_003156187.1 NZ_CP125660:518544-518894 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT282249 WP_003225183.1 NZ_CP125660:518258-518539 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References