Detailed information of TA system TA028221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 454635..455162
• Replicon: chromosome
• Accession: NC_018525
• Organism: Pectobacterium carotovorum subsp. carotovorum PCC21

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: PCC21_RS02090
• Protein ID: WP_014913960.1
• Coordinates: 454887..455162 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: PCC21_RS02085
• Protein ID: WP_043901796.1
• Coordinates: 454635..454883 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCC21_RS02060	450308..451384	-	1077	WP_014913954.1	serine/threonine protein kinase	-
PCC21_RS02065	451381..452439	-	1059	WP_014913955.1	serine/threonine protein kinase	-
PCC21_RS02070	452429..453593	-	1165	Protein_397	hypothetical protein	-
PCC21_RS02075	453791..454027	+	237	WP_014913957.1	hypothetical protein	-
PCC21_RS21330	453921..454169	-	249	WP_071822562.1	ogr/Delta-like zinc finger family protein	-
PCC21_RS02080	454166..454435	-	270	Protein_400	capsid size determination protein	-
PCC21_RS02085	454635..454883	+	249	WP_043901796.1	ribbon-helix-helix domain-containing protein	Antitoxin
PCC21_RS02090	454887..455162	+	276	WP_014913960.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PCC21_RS02095	455210..455722	+	513	WP_014913961.1	AAA family ATPase	-
PCC21_RS02100	456039..456521	-	483	WP_014913962.1	hypothetical protein	-
PCC21_RS02105	456532..457236	-	705	WP_014913963.1	hypothetical protein	-
PCC21_RS02110	457259..458791	-	1533	WP_014913964.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-
PCC21_RS02115	458781..460013	-	1233	WP_014913965.1	FtsX-like permease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 11045.28 Da
• Isoelectric Point: 4.8888

>T28221 WP_014913960.1 NC_018525:454887-455162 [Pectobacterium carotovorum subsp. carotovorum PCC21]
MEIRWTQKAQDDLERIYGFASQYSRQHADEVLDRLIIGTTGLADYPRIGVSQTRYEPREVRKILFDDYEVHYEIQHDTIY
IVDLWHTREDR

Nucleotide

• Length: 276 bp

>T28221 NC_018525:454887-455162 [Pectobacterium carotovorum subsp. carotovorum PCC21]
ATGGAAATACGTTGGACGCAAAAGGCACAGGATGACCTTGAACGTATTTATGGGTTTGCCAGCCAGTACAGTCGGCAACA
TGCTGACGAGGTGTTAGATCGTTTGATCATCGGCACCACGGGGCTTGCTGACTACCCGAGAATCGGCGTATCTCAGACAA
GATATGAACCACGTGAGGTGAGAAAGATTTTGTTTGATGATTATGAAGTTCACTATGAAATTCAGCATGATACGATTTAT
ATCGTCGATCTGTGGCATACACGAGAAGACCGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9048.44 Da
• Isoelectric Point: 8.7560

>AT28221 WP_043901796.1 NC_018525:454635-454883 [Pectobacterium carotovorum subsp. carotovorum PCC21]
MGQPAKQTISAQIPAELAAAVERLAIELDRSKSWVIKEALTAMIEERERRHQQVLKGLADVDAGRVVSHADVIDFANKLK
KS

Nucleotide

• Length: 249 bp

>AT28221 NC_018525:454635-454883 [Pectobacterium carotovorum subsp. carotovorum PCC21]
ATGGGACAACCCGCGAAACAGACGATCAGTGCTCAGATACCCGCCGAACTTGCGGCTGCGGTTGAAAGACTGGCTATTGA
ATTGGATAGATCCAAAAGCTGGGTGATTAAAGAAGCGCTGACCGCAATGATTGAAGAGAGAGAGCGGCGGCATCAGCAGG
TTTTGAAAGGTCTTGCTGATGTCGATGCGGGAAGAGTAGTAAGCCATGCAGATGTGATCGACTTCGCCAACAAACTGAAA
AAGTCATAA