Detailed information of TA system TA028220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 411649..412422
• Replicon: chromosome
• Accession: NC_018525
• Organism: Pectobacterium carotovorum subsp. carotovorum PCC21

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: PCC21_RS21325
• Protein ID: WP_071822561.1
• Coordinates: 412126..412422 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PCC21_RS01885
• Protein ID: WP_014913924.1
• Coordinates: 411649..412122 (-)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCC21_RS01870	406971..407894	-	924	WP_043901795.1	aminoimidazole riboside kinase	-
PCC21_RS01875	408354..408905	+	552	WP_014913922.1	polymer-forming cytoskeletal protein	-
PCC21_RS01880	408942..411659	+	2718	WP_014913923.1	CRISPR-associated helicase/endonuclease Cas3	-
PCC21_RS01885	411649..412122	-	474	WP_014913924.1	transcriptional regulator	Antitoxin
PCC21_RS21325	412126..412422	-	297	WP_071822561.1	type II toxin-antitoxin system HigB family toxin	Toxin
PCC21_RS01890	413100..414668	+	1569	WP_014913926.1	type I-E CRISPR-associated protein Cse1/CasA	-
PCC21_RS01895	414652..415299	+	648	WP_014913927.1	type I-E CRISPR-associated protein Cse2/CasB	-
PCC21_RS01900	415303..416370	+	1068	WP_014913928.1	type I-E CRISPR-associated protein Cas7/Cse4/CasC	-
PCC21_RS01905	416380..417114	+	735	WP_014913929.1	type I-E CRISPR-associated protein Cas5/CasD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (52-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (100-155)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11780.46 Da
• Isoelectric Point: 8.1314

>T28220 WP_071822561.1 NC_018525:c412422-412126 [Pectobacterium carotovorum subsp. carotovorum PCC21]
MKLWLDVFDKPSLRFESYEQLCHVWRNTSCWNVDRIPFTLLENESRKGPLDIYVFDIHGNNCRIIAWLNPNTGSLYVKDV
CSHAEYDKWWKAQTKTKR

Nucleotide

• Length: 297 bp

>T28220 NC_018525:c412422-412126 [Pectobacterium carotovorum subsp. carotovorum PCC21]
TTGAAGTTATGGCTGGATGTTTTTGACAAGCCATCACTGCGGTTTGAGTCCTACGAACAGTTATGTCATGTATGGCGAAA
TACATCTTGCTGGAATGTCGATCGCATACCTTTCACATTGCTAGAAAACGAAAGCCGCAAAGGACCACTTGATATATATG
TATTTGATATACATGGTAACAATTGCAGAATCATAGCGTGGTTAAACCCTAATACGGGTTCTTTGTATGTGAAAGACGTG
TGCTCTCACGCCGAGTACGATAAGTGGTGGAAAGCTCAAACGAAGACAAAAAGGTGA

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17863.22 Da
• Isoelectric Point: 4.2700

>AT28220 WP_014913924.1 NC_018525:c412122-411649 [Pectobacterium carotovorum subsp. carotovorum PCC21]
MRNTSLGSNSPVGISGLIENLPLLLQEASEQLNSLSVLLTACMEPIEDDKDLQERMECINQLYLYSSDVNDIPATFAELI
ADRVYEYEKKNRDVPHVSQAEALAFFIEDRRLKQSDLKQIATQSVISDIINGKRTMTLQQVKDFSRYFNVPVETFID

Nucleotide

• Length: 474 bp

>AT28220 NC_018525:c412122-411649 [Pectobacterium carotovorum subsp. carotovorum PCC21]
ATGCGAAATACATCTCTGGGTTCTAACAGTCCTGTAGGAATCAGCGGGCTCATCGAAAATTTACCTCTTCTGCTTCAGGA
AGCCTCTGAGCAGCTTAACAGCCTGTCTGTTTTGCTCACTGCCTGTATGGAACCTATTGAAGACGATAAAGATCTTCAGG
AAAGAATGGAGTGCATCAATCAGCTTTACCTCTACTCTTCTGATGTCAATGATATTCCAGCCACTTTCGCAGAGCTCATC
GCAGACAGAGTCTATGAATATGAAAAGAAAAATCGGGATGTGCCCCACGTCAGTCAGGCTGAAGCCTTAGCTTTTTTTAT
TGAAGACAGAAGGCTGAAACAGTCAGATTTAAAGCAAATTGCAACACAAAGCGTCATCTCTGACATTATTAACGGTAAGC
GAACCATGACATTACAGCAAGTTAAGGACTTTTCTCGCTACTTTAATGTGCCTGTTGAGACATTTATTGACTGA