Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1232165..1232796 Replicon chromosome
Accession NZ_CP125620
Organism Mycobacterium tuberculosis strain BLR 4299 2019

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag O3Q54_RS05895 Protein ID WP_003898728.1
Coordinates 1232165..1232476 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag O3Q54_RS05900 Protein ID WP_003405836.1
Coordinates 1232476..1232796 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O3Q54_RS05875 (O3Q54_05875) 1227646..1229070 - 1425 WP_003405805.1 class II fumarate hydratase -
O3Q54_RS05880 (O3Q54_05880) 1229101..1230189 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
O3Q54_RS05885 (O3Q54_05885) 1230245..1230889 + 645 WP_003915494.1 DUF4245 domain-containing protein -
O3Q54_RS05890 (O3Q54_05890) 1230896..1232053 - 1158 WP_003898727.1 AI-2E family transporter -
O3Q54_RS05895 (O3Q54_05895) 1232165..1232476 - 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
O3Q54_RS05900 (O3Q54_05900) 1232476..1232796 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
O3Q54_RS05905 (O3Q54_05905) 1232806..1234342 + 1537 Protein_1163 carboxylesterase/lipase family protein -
O3Q54_RS05910 (O3Q54_05910) 1234349..1235461 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
O3Q54_RS05915 (O3Q54_05915) 1235471..1235728 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
O3Q54_RS05920 (O3Q54_05920) 1235718..1236965 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
O3Q54_RS05925 (O3Q54_05925) 1236962..1237600 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T282182 WP_003898728.1 NZ_CP125620:c1232476-1232165 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT282182 WP_003405836.1 NZ_CP125620:c1232796-1232476 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References