Detailed information of TA system TA282153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 5676641..5678370
• Replicon: chromosome
• Accession: NZ_CP125618
• Organism: Pseudomonas sp. PMCC200344

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: QFW87_RS26045
• Protein ID: WP_283189210.1
• Coordinates: 5676641..5677285 (+)
• Length: 215 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: QFW87_RS26050
• Protein ID: WP_283189211.1
• Coordinates: 5677282..5678370 (+)
• Length: 363 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QFW87_RS26010	5671822..5672634	+	813	WP_283189204.1	zinc-dependent peptidase	-
QFW87_RS26015	5672748..5673275	+	528	WP_028620880.1	inorganic diphosphatase	-
QFW87_RS26020	5673499..5674251	+	753	WP_283189205.1	S24 family peptidase	-
QFW87_RS26025	5674841..5675185	-	345	WP_283189206.1	hypothetical protein	-
QFW87_RS26030	5675236..5675898	+	663	WP_283189207.1	restriction endonuclease	-
QFW87_RS26035	5676065..5676226	+	162	WP_283189208.1	hypothetical protein	-
QFW87_RS26040	5676282..5676644	+	363	WP_283189209.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
QFW87_RS26045	5676641..5677285	+	645	WP_283189210.1	DUF4433 domain-containing protein	Toxin
QFW87_RS26050	5677282..5678370	+	1089	WP_283189211.1	macro domain-containing protein	Antitoxin
QFW87_RS26055	5678504..5679319	-	816	WP_283189212.1	DUF2135 domain-containing protein	-
QFW87_RS26060	5679323..5680942	-	1620	WP_283189213.1	DUF2300 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (14-213)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 215 a.a.
• Molecular weight: 24952.52 Da
• Isoelectric Point: 6.9121

>T282153 WP_283189210.1 NZ_CP125618:5676641-5677285 [Pseudomonas sp. PMCC200344]
MNYTSLNPEKALIWRIIHRDNLPWILDNGLHCANSKVLAPQYVNIGNVDLIDKRRSRRVPIAPEGFLADYVPFYFTPFSV
MMKNIHSGWSVQQRKNDEIVILVSSLYRVEELGLPFVFTNAHAYPDWTDYYSDLANLGEIDWSILQRRDFKRDPDDPRKM
ERYQAEALIHHHLPITGLLGIMCYSDAMKERIEQDVAARGLTLSVHARSGWYFQ

Nucleotide

• Length: 645 bp

Antitoxin

Protein

• Length: 363 a.a.
• Molecular weight: 40485.37 Da
• Isoelectric Point: 5.7357

>AT282153 WP_283189211.1 NZ_CP125618:5677282-5678370 [Pseudomonas sp. PMCC200344]
MIRFTQGNLLEAKTEALVNTVNTVGVMGKGIALMFKERFAENYRLYSAACKAGAVETGKVHVTAVNELDGPRWIVNFPTK
RHWRSPSQMAWITEGLQDLRRFLIENDVKSVAVPPLGAGNGGLKWPEVREQIVDVLSDLDVDVLVFEPSNQYLNVAKRSG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIEKLPDTENPLNLKFVAHKYGPYANRLEHLLNNLDGSYLH
CDKRISDAGISDVIWFDQDRKAFLQTYLKTEAKEYSQALERTAELIDGFESPFGMELLATVDWLLNQEGVSPTVPAVREA
LSHWDGGASAAARKSKLFDDQALDIALTRLTSSSFGPVMASC

Nucleotide

• Length: 1089 bp