Detailed information of TA system TA282133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1637150..1638845
• Replicon: chromosome
• Accession: NZ_CP125365
• Organism: Pseudomonas aeruginosa strain ZY36

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: QMK42_RS07675
• Protein ID: WP_003112901.1
• Coordinates: 1637150..1638019 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: QMK42_RS07680
• Protein ID: WP_003112902.1
• Coordinates: 1638012..1638845 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMK42_RS07650 (QMK42_07650)	1633158..1634009	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
QMK42_RS07655 (QMK42_07655)	1634050..1635057	+	1008	WP_003162724.1	ABC transporter substrate-binding protein	-
QMK42_RS07660 (QMK42_07660)	1635054..1635830	+	777	WP_003112898.1	ABC transporter permease	-
QMK42_RS07665 (QMK42_07665)	1635836..1636597	+	762	WP_003112899.1	SDR family oxidoreductase	-
QMK42_RS07670 (QMK42_07670)	1636611..1637141	+	531	WP_003112900.1	cupin domain-containing protein	-
QMK42_RS07675 (QMK42_07675)	1637150..1638019	+	870	WP_003112901.1	alpha/beta hydrolase	Toxin
QMK42_RS07680 (QMK42_07680)	1638012..1638845	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
QMK42_RS07685 (QMK42_07685)	1638832..1639629	+	798	WP_017001845.1	SDR family oxidoreductase	-
QMK42_RS07690 (QMK42_07690)	1639622..1641304	+	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
QMK42_RS07695 (QMK42_07695)	1641315..1642118	+	804	WP_003453564.1	aspartate dehydrogenase	-
QMK42_RS07700 (QMK42_07700)	1642130..1643614	+	1485	WP_031756606.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T282133 WP_003112901.1 NZ_CP125365:1637150-1638019 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT282133 WP_003112902.1 NZ_CP125365:1638012-1638845 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1