Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5388028..5388653 Replicon chromosome
Accession NZ_CP125343
Organism Klebsiella pneumoniae strain WYKP589

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag QJR23_RS28005 Protein ID WP_002882817.1
Coordinates 5388028..5388411 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag QJR23_RS28010 Protein ID WP_004150355.1
Coordinates 5388411..5388653 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJR23_RS27990 (QJR23_27990) 5385394..5386296 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
QJR23_RS27995 (QJR23_27995) 5386293..5386928 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
QJR23_RS28000 (QJR23_28000) 5386925..5387854 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
QJR23_RS28005 (QJR23_28005) 5388028..5388411 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
QJR23_RS28010 (QJR23_28010) 5388411..5388653 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
QJR23_RS28015 (QJR23_28015) 5388858..5389775 + 918 WP_002882812.1 alpha/beta hydrolase -
QJR23_RS28020 (QJR23_28020) 5389789..5390730 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
QJR23_RS28025 (QJR23_28025) 5390775..5391212 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
QJR23_RS28030 (QJR23_28030) 5391209..5392069 - 861 WP_002882807.1 virulence factor BrkB family protein -
QJR23_RS28035 (QJR23_28035) 5392063..5392662 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T282051 WP_002882817.1 NZ_CP125343:c5388411-5388028 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT282051 WP_004150355.1 NZ_CP125343:c5388653-5388411 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References