Detailed information of TA system TA028201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 327604..328190
• Replicon: chromosome
• Accession: NC_018522
• Organism: Klebsiella pneumoniae subsp. pneumoniae 1084

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A330WAT7
• Locus tag: A79E_RS01555
• Protein ID: WP_014906830.1
• Coordinates: 327822..328190 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: A79E_RS01550
• Protein ID: WP_004174006.1
• Coordinates: 327604..327825 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A79E_RS01530	323761..324687	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
A79E_RS01535	324684..325961	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
A79E_RS01540	325958..326725	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
A79E_RS01545	326727..327440	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
A79E_RS01550	327604..327825	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
A79E_RS01555	327822..328190	+	369	WP_014906830.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
A79E_RS01560	328462..329778	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
A79E_RS01565	329885..330772	+	888	WP_014906831.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
A79E_RS01570	330769..331614	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
A79E_RS01575	331616..332686	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	322590..333423	10833

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (28-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13537.95 Da
• Isoelectric Point: 8.6410

>T28201 WP_014906830.1 NC_018522:327822-328190 [Klebsiella pneumoniae subsp. pneumoniae 1084]
MTLQIISAEEIILFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T28201 NC_018522:327822-328190 [Klebsiella pneumoniae subsp. pneumoniae 1084]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACTGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT28201 WP_004174006.1 NC_018522:327604-327825 [Klebsiella pneumoniae subsp. pneumoniae 1084]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT28201 NC_018522:327604-327825 [Klebsiella pneumoniae subsp. pneumoniae 1084]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A330WAT7

Antitoxin

• AlphaFold DB: A0A5E5YJY7