Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2498018..2498669 Replicon chromosome
Accession NZ_CP125315
Organism Lacticaseibacillus rhamnosus strain k32

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag QJQ50_RS11590 Protein ID WP_005686631.1
Coordinates 2498018..2498401 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K8QH19
Locus tag QJQ50_RS11595 Protein ID WP_005686632.1
Coordinates 2498421..2498669 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJQ50_RS11585 (QJQ50_11585) 2496771..2497298 - 528 WP_032964074.1 QueT transporter family protein -
QJQ50_RS11590 (QJQ50_11590) 2498018..2498401 - 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
QJQ50_RS11595 (QJQ50_11595) 2498421..2498669 - 249 WP_005686632.1 antitoxin Antitoxin
QJQ50_RS11600 (QJQ50_11600) 2498781..2499920 - 1140 WP_032964346.1 alanine racemase -
QJQ50_RS11605 (QJQ50_11605) 2499907..2500281 - 375 WP_032964345.1 holo-ACP synthase -
QJQ50_RS11610 (QJQ50_11610) 2500450..2501958 - 1509 WP_032964344.1 DEAD/DEAH box helicase -
QJQ50_RS11615 (QJQ50_11615) 2502232..2503620 - 1389 WP_032964374.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T282004 WP_005686631.1 NZ_CP125315:c2498401-2498018 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8936.13 Da        Isoelectric Point: 4.4778

>AT282004 WP_005686632.1 NZ_CP125315:c2498669-2498421 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB K8QH19

References