Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 3653240..3654156 Replicon chromosome
Accession NZ_CP125292
Organism Bacillus subtilis strain SRCM117797

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag QL281_RS19540 Protein ID WP_003244695.1
Coordinates 3653410..3654156 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag QL281_RS19535 Protein ID WP_003232646.1
Coordinates 3653240..3653410 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QL281_RS19500 (3650120) 3650120..3650432 + 313 Protein_3781 XkdW family protein -
QL281_RS19505 (3650429) 3650429..3650593 + 165 WP_003232658.1 XkdX family protein -
QL281_RS19510 (3650637) 3650637..3651476 + 840 WP_041351214.1 phage-like element PBSX protein XepA -
QL281_RS19515 (3651529) 3651529..3651798 + 270 WP_041351215.1 hemolysin XhlA family protein -
QL281_RS19520 (3651811) 3651811..3652074 + 264 WP_041351216.1 phage holin -
QL281_RS19525 (3652087) 3652087..3652980 + 894 WP_041351217.1 N-acetylmuramoyl-L-alanine amidase -
QL281_RS19530 (3653017) 3653017..3653154 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
QL281_RS19535 (3653240) 3653240..3653410 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
QL281_RS19540 (3653410) 3653410..3654156 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
QL281_RS19545 (3654266) 3654266..3655267 - 1002 WP_014479569.1 inorganic phosphate transporter -
QL281_RS19550 (3655280) 3655280..3655897 - 618 WP_003218470.1 DUF47 domain-containing protein -
QL281_RS19555 (3656173) 3656173..3657489 - 1317 WP_144458963.1 serine/threonine exchanger -
QL281_RS19560 (3657878) 3657878..3658828 + 951 WP_014476561.1 ring-cleaving dioxygenase -
QL281_RS19565 (3658937) 3658937..3659038 + 102 Protein_3794 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T281968 WP_003244695.1 NZ_CP125292:c3654156-3653410 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT281968 WP_003232646.1 NZ_CP125292:c3653410-3653240 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References