Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2831195..2831831 Replicon chromosome
Accession NZ_CP125292
Organism Bacillus subtilis strain SRCM117797

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QL281_RS15180 Protein ID WP_003156187.1
Coordinates 2831481..2831831 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag QL281_RS15175 Protein ID WP_003225183.1
Coordinates 2831195..2831476 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QL281_RS15155 (2827555) 2827555..2828154 - 600 WP_041351790.1 rhomboid family intramembrane serine protease -
QL281_RS15160 (2828249) 2828249..2828614 + 366 WP_014475874.1 holo-ACP synthase -
QL281_RS15165 (2828780) 2828780..2829796 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
QL281_RS15170 (2829910) 2829910..2831079 + 1170 WP_017697002.1 alanine racemase -
QL281_RS15175 (2831195) 2831195..2831476 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QL281_RS15180 (2831481) 2831481..2831831 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QL281_RS15185 (2831946) 2831946..2832770 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
QL281_RS15190 (2832775) 2832775..2833140 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
QL281_RS15195 (2833144) 2833144..2833545 + 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
QL281_RS15200 (2833557) 2833557..2834564 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
QL281_RS15205 (2834626) 2834626..2834955 + 330 WP_014662855.1 anti-sigma factor antagonist RsbV -
QL281_RS15210 (2834952) 2834952..2835434 + 483 WP_003234299.1 anti-sigma B factor RsbW -
QL281_RS15215 (2835400) 2835400..2836188 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
QL281_RS15220 (2836188) 2836188..2836787 + 600 WP_017696999.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T281967 WP_003156187.1 NZ_CP125292:2831481-2831831 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT281967 WP_003225183.1 NZ_CP125292:2831195-2831476 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References