Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 480567..481204 Replicon chromosome
Accession NZ_CP125289
Organism Bacillus velezensis strain SF334

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QLX65_RS02495 Protein ID WP_003156187.1
Coordinates 480854..481204 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag QLX65_RS02490 Protein ID WP_003156188.1
Coordinates 480567..480848 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QLX65_RS02470 (QLX65_02470) 476932..477531 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
QLX65_RS02475 (QLX65_02475) 477624..477989 + 366 WP_014304402.1 holo-ACP synthase -
QLX65_RS02480 (QLX65_02480) 478154..479161 + 1008 WP_283002502.1 outer membrane lipoprotein carrier protein LolA -
QLX65_RS02485 (QLX65_02485) 479278..480447 + 1170 WP_003156189.1 alanine racemase -
QLX65_RS02490 (QLX65_02490) 480567..480848 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QLX65_RS02495 (QLX65_02495) 480854..481204 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QLX65_RS02500 (QLX65_02500) 481322..482143 + 822 WP_003156182.1 STAS domain-containing protein -
QLX65_RS02505 (QLX65_02505) 482148..482513 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
QLX65_RS02510 (QLX65_02510) 482516..482917 + 402 WP_003156178.1 anti-sigma regulatory factor -
QLX65_RS02515 (QLX65_02515) 482929..483936 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
QLX65_RS02520 (QLX65_02520) 484000..484329 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
QLX65_RS02525 (QLX65_02525) 484326..484808 + 483 WP_003156173.1 anti-sigma B factor RsbW -
QLX65_RS02530 (QLX65_02530) 484774..485562 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
QLX65_RS02535 (QLX65_02535) 485562..486164 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T281961 WP_003156187.1 NZ_CP125289:480854-481204 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT281961 WP_003156188.1 NZ_CP125289:480567-480848 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References