281953

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 477058..477695 Replicon chromosome
Accession NZ_CP125283
Organism Bacillus velezensis strain IFST-221

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QLX50_RS02430 Protein ID WP_003156187.1
Coordinates 477345..477695 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag QLX50_RS02425 Protein ID WP_003156188.1
Coordinates 477058..477339 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QLX50_RS02405 (QLX50_02405) 473423..474022 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
QLX50_RS02410 (QLX50_02410) 474115..474480 + 366 WP_007609580.1 holo-ACP synthase -
QLX50_RS02415 (QLX50_02415) 474645..475652 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
QLX50_RS02420 (QLX50_02420) 475769..476938 + 1170 WP_044802654.1 alanine racemase -
QLX50_RS02425 (QLX50_02425) 477058..477339 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QLX50_RS02430 (QLX50_02430) 477345..477695 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QLX50_RS02435 (QLX50_02435) 477813..478634 + 822 WP_003156182.1 STAS domain-containing protein -
QLX50_RS02440 (QLX50_02440) 478639..479004 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
QLX50_RS02445 (QLX50_02445) 479007..479408 + 402 WP_003156178.1 anti-sigma regulatory factor -
QLX50_RS02450 (QLX50_02450) 479420..480427 + 1008 WP_044802655.1 PP2C family protein-serine/threonine phosphatase -
QLX50_RS02455 (QLX50_02455) 480491..480820 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
QLX50_RS02460 (QLX50_02460) 480817..481299 + 483 WP_007609591.1 anti-sigma B factor RsbW -
QLX50_RS02465 (QLX50_02465) 481265..482053 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
QLX50_RS02470 (QLX50_02470) 482053..482655 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T281953 WP_003156187.1 NZ_CP125283:477345-477695 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT281953 WP_003156188.1 NZ_CP125283:477058-477339 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References