Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5247963..5248588 Replicon chromosome
Accession NZ_CP125088
Organism Klebsiella pneumoniae strain BAK085

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag QJS47_RS25840 Protein ID WP_002882817.1
Coordinates 5247963..5248346 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag QJS47_RS25845 Protein ID WP_004150355.1
Coordinates 5248346..5248588 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJS47_RS25825 (QJS47_25825) 5245329..5246231 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
QJS47_RS25830 (QJS47_25830) 5246228..5246863 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
QJS47_RS25835 (QJS47_25835) 5246860..5247789 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
QJS47_RS25840 (QJS47_25840) 5247963..5248346 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
QJS47_RS25845 (QJS47_25845) 5248346..5248588 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
QJS47_RS25850 (QJS47_25850) 5248793..5249710 + 918 WP_002882812.1 alpha/beta hydrolase -
QJS47_RS25855 (QJS47_25855) 5249724..5250665 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
QJS47_RS25860 (QJS47_25860) 5250710..5251147 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
QJS47_RS25865 (QJS47_25865) 5251144..5252004 - 861 WP_002882807.1 virulence factor BrkB family protein -
QJS47_RS25870 (QJS47_25870) 5251998..5252597 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T281911 WP_002882817.1 NZ_CP125088:c5248346-5247963 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT281911 WP_004150355.1 NZ_CP125088:c5248588-5248346 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References