Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3874024..3874649 Replicon chromosome
Accession NZ_CP125083
Organism Klebsiella pneumoniae subsp. pneumoniae strain HVKP1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag QFB57_RS19445 Protein ID WP_002882817.1
Coordinates 3874024..3874407 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag QFB57_RS19450 Protein ID WP_004150355.1
Coordinates 3874407..3874649 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QFB57_RS19430 (3871390) 3871390..3872292 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
QFB57_RS19435 (3872289) 3872289..3872924 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
QFB57_RS19440 (3872921) 3872921..3873850 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
QFB57_RS19445 (3874024) 3874024..3874407 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
QFB57_RS19450 (3874407) 3874407..3874649 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
QFB57_RS19455 (3874854) 3874854..3875771 + 918 WP_004181612.1 alpha/beta hydrolase -
QFB57_RS19460 (3875785) 3875785..3876726 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
QFB57_RS19465 (3876771) 3876771..3877208 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
QFB57_RS19470 (3877205) 3877205..3878065 - 861 WP_004146232.1 virulence factor BrkB family protein -
QFB57_RS19475 (3878059) 3878059..3878658 - 600 WP_004146231.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T281877 WP_002882817.1 NZ_CP125083:c3874407-3874024 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT281877 WP_004150355.1 NZ_CP125083:c3874649-3874407 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References