28183

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1327941..1328857 Replicon chromosome
Accession NC_018520
Organism Bacillus subtilis QB928

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag B657_RS07065 Protein ID WP_003244695.1
Coordinates 1328111..1328857 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag B657_RS07060 Protein ID WP_003232646.1
Coordinates 1327941..1328111 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B657_RS07030 1324804..1325133 + 330 WP_003232660.1 phage-like element PBSX protein XkdW -
B657_RS07035 1325130..1325294 + 165 WP_003232658.1 XkdX family protein -
B657_RS07040 1325338..1326177 + 840 WP_003245597.1 phage-like element PBSX protein XepA -
B657_RS07045 1326230..1326499 + 270 WP_003232655.1 hemolysin XhlA family protein -
B657_RS07050 1326512..1326775 + 264 WP_003232653.1 phage holin -
B657_RS07055 1326788..1327681 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
B657_RS22380 1327718..1327855 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
B657_RS07060 1327941..1328111 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
B657_RS07065 1328111..1328857 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
B657_RS07070 1328967..1329968 - 1002 WP_003232642.1 inorganic phosphate transporter -
B657_RS07075 1329981..1330598 - 618 WP_003218470.1 DUF47 domain-containing protein -
B657_RS07080 1330874..1332190 - 1317 WP_003244819.1 serine/threonine exchanger -
B657_RS07085 1332579..1333529 + 951 WP_003245086.1 ring-cleaving dioxygenase -
B657_RS22385 1333630..1333776 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-238)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T28183 WP_003244695.1 NC_018520:c1328857-1328111 [Bacillus subtilis QB928]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T28183 NC_018520:c1328857-1328111 [Bacillus subtilis QB928]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT28183 WP_003232646.1 NC_018520:c1328111-1327941 [Bacillus subtilis QB928]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT28183 NC_018520:c1328111-1327941 [Bacillus subtilis QB928]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 3O6Q


Antitoxin

Source ID Structure
AlphaFold DB G4EXD8

References