Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518667..519303 Replicon chromosome
Accession NC_018520
Organism Bacillus subtilis QB928

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag B657_RS02685 Protein ID WP_003156187.1
Coordinates 518953..519303 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag B657_RS02680 Protein ID WP_003225183.1
Coordinates 518667..518948 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B657_RS02660 515027..515626 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
B657_RS02665 515721..516086 + 366 WP_003234281.1 holo-ACP synthase -
B657_RS02670 516252..517268 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
B657_RS02675 517383..518551 + 1169 Protein_473 alanine racemase -
B657_RS02680 518667..518948 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
B657_RS02685 518953..519303 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
B657_RS02690 519418..520242 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
B657_RS02695 520247..520612 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
B657_RS02700 520616..521017 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
B657_RS02705 521029..522036 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
B657_RS02710 522098..522427 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
B657_RS02715 522424..522906 + 483 WP_003234299.1 anti-sigma B factor RsbW -
B657_RS02720 522872..523660 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
B657_RS02725 523660..524259 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T28182 WP_003156187.1 NC_018520:518953-519303 [Bacillus subtilis QB928]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T28182 NC_018520:518953-519303 [Bacillus subtilis QB928]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT28182 WP_003225183.1 NC_018520:518667-518948 [Bacillus subtilis QB928]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT28182 NC_018520:518667-518948 [Bacillus subtilis QB928]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References