Detailed information of TA system TA028179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2978870..2979435
• Replicon: chromosome
• Accession: NC_018514
• Organism: Burkholderia cepacia GG4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GEM_RS29035
• Protein ID: WP_014901017.1
• Coordinates: 2978870..2979241 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B1FGI9
• Locus tag: GEM_RS30820
• Protein ID: WP_006752328.1
• Coordinates: 2979238..2979435 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEM_RS29020	2974746..2975471	+	726	WP_014901014.1	site-2 protease family protein	-
GEM_RS29025	2975531..2976874	-	1344	WP_187293285.1	MFS transporter	-
GEM_RS29030	2977154..2978731	+	1578	WP_014901016.1	aldehyde dehydrogenase (NADP(+))	-
GEM_RS29035	2978870..2979241	-	372	WP_014901017.1	PIN domain-containing protein	Toxin
GEM_RS30820	2979238..2979435	-	198	WP_006752328.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GEM_RS29040	2979624..2980175	+	552	WP_014901018.1	hypothetical protein	-
GEM_RS29045	2980313..2981227	-	915	WP_014901019.1	5-dehydro-4-deoxyglucarate dehydratase	-
GEM_RS29050	2981458..2983011	+	1554	WP_014901020.1	galactarate dehydratase	-
GEM_RS29055	2983192..2983584	+	393	WP_014901021.1	nuclear transport factor 2 family protein	-
GEM_RS29060	2983664..2984119	+	456	WP_014901022.1	RbsD/FucU family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-46)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13675.74 Da
• Isoelectric Point: 5.1342

>T28179 WP_014901017.1 NC_018514:c2979241-2978870 [Burkholderia cepacia GG4]
MILVDTSVWIDHLRAGDATLTTLLEAERELIHPFIIGELALGSMRDRQTVLDALRDLPAATPATDDEVQRMIDVVPLHGL
DIGYVDAHLLASLRLTGDSKLWTRDRRLLAAAERLQLVTHPAH

Nucleotide

• Length: 372 bp

>T28179 NC_018514:c2979241-2978870 [Burkholderia cepacia GG4]
ATGATTCTTGTCGACACTTCAGTGTGGATCGACCACCTGCGGGCCGGTGACGCAACGCTCACGACATTGCTGGAAGCTGA
ACGGGAGCTGATCCATCCGTTCATCATCGGCGAGCTTGCGCTCGGCAGCATGCGCGATCGCCAGACCGTACTGGATGCGC
TGCGCGACCTTCCCGCCGCCACCCCGGCAACCGACGACGAAGTCCAGCGCATGATCGATGTCGTACCGCTTCACGGGCTA
GACATCGGTTACGTCGACGCACATCTGCTTGCCTCGCTCAGACTGACCGGAGACAGCAAGCTGTGGACACGCGACCGGCG
CCTGCTGGCCGCAGCCGAGCGCCTGCAACTTGTCACTCACCCCGCTCACTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7022.04 Da
• Isoelectric Point: 10.6826

>AT28179 WP_006752328.1 NC_018514:c2979435-2979238 [Burkholderia cepacia GG4]
VRTTLSLDDALLAKAQQLTGVTEKSALVREALRALIARESARRLARLGGTEPDLESVPRRPSEPA

Nucleotide

• Length: 198 bp

>AT28179 NC_018514:c2979435-2979238 [Burkholderia cepacia GG4]
GTGCGCACCACCTTGTCCCTCGACGACGCACTGCTCGCCAAAGCGCAGCAATTAACCGGCGTCACCGAAAAATCGGCGCT
GGTGAGAGAAGCGCTGCGCGCATTGATCGCGCGCGAAAGCGCACGACGACTCGCCCGCCTCGGCGGTACCGAGCCCGACC
TTGAATCTGTTCCGCGCCGCCCATCGGAGCCCGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A2S9KMB8