Detailed information of TA system TA028176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 1170622..1171255
• Replicon: chromosome
• Accession: NC_018514
• Organism: Burkholderia cepacia GG4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GEM_RS20995
• Protein ID: WP_014899398.1
• Coordinates: 1170622..1171035 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GEM_RS21000
• Protein ID: WP_014899399.1
• Coordinates: 1171022..1171255 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEM_RS20980	1166060..1167187	-	1128	WP_014899394.1	VOC family protein	-
GEM_RS20985	1167545..1168852	+	1308	WP_014899395.1	shikimate transporter	-
GEM_RS20990	1169066..1170367	+	1302	WP_014899396.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
GEM_RS20995	1170622..1171035	-	414	WP_014899398.1	PIN domain-containing protein	Toxin
GEM_RS21000	1171022..1171255	-	234	WP_014899399.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GEM_RS21005	1171423..1172193	+	771	WP_014899400.1	helix-turn-helix domain-containing protein	-
GEM_RS21010	1172248..1174548	-	2301	WP_014899401.1	ATP-dependent DNA helicase	-
GEM_RS21015	1174545..1176239	-	1695	WP_014899402.1	VRR-NUC domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-117)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15372.63 Da
• Isoelectric Point: 4.9532

>T28176 WP_014899398.1 NC_018514:c1171035-1170622 [Burkholderia cepacia GG4]
MSVIESAKSFVDSNVVLYLLSEDETKANRSEALFLQRPTISVQVLNEVASVCSRKLRMPWSDVGQFLEPIRRLCEVVPIT
VDTHDLARRLAERHGLSFYDACIVATASIEGCEYLYTEAMHDGLRVEGGPVLRNPFI

Nucleotide

• Length: 414 bp

>T28176 NC_018514:c1171035-1170622 [Burkholderia cepacia GG4]
ATGAGCGTGATTGAGTCGGCGAAATCGTTCGTCGACAGCAATGTCGTGCTTTATCTGCTGTCCGAGGACGAAACCAAGGC
CAACCGTTCGGAGGCGCTGTTCCTTCAGCGGCCGACCATTAGCGTCCAGGTACTGAACGAAGTTGCCAGCGTTTGCAGCC
GCAAATTGCGGATGCCGTGGAGCGACGTGGGGCAGTTTTTGGAGCCGATCCGGCGTTTGTGCGAAGTCGTGCCGATCACG
GTCGACACACATGATCTGGCGCGAAGGTTGGCGGAGCGGCACGGCTTGTCCTTCTACGATGCCTGCATCGTGGCAACTGC
TTCGATCGAGGGTTGCGAATACTTGTACACCGAGGCCATGCACGACGGCTTGCGCGTGGAGGGCGGTCCAGTGTTGCGAA
ATCCATTTATCTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8819.07 Da
• Isoelectric Point: 8.8306

>AT28176 WP_014899399.1 NC_018514:c1171255-1171022 [Burkholderia cepacia GG4]
MQVAKWGNSLAVRLPASVVDALELREGDDIEIVVDAPRVFAVSRKPRPDELLQRLRRFRGKLPADFKFSRDDANERD

Nucleotide

• Length: 234 bp

>AT28176 NC_018514:c1171255-1171022 [Burkholderia cepacia GG4]
ATGCAGGTTGCAAAGTGGGGCAATAGCTTGGCGGTCCGCCTTCCAGCCAGCGTGGTCGATGCACTGGAGCTCCGAGAAGG
GGACGATATCGAAATTGTGGTCGACGCCCCTCGCGTTTTCGCGGTAAGTCGCAAGCCGCGCCCCGACGAGTTGCTGCAGC
GGCTGAGGCGTTTCAGGGGCAAGCTGCCCGCCGATTTCAAGTTCAGCCGAGACGACGCAAATGAGCGTGATTGA