Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 5274120..5274762 Replicon chromosome
Accession NC_018508
Organism Bacillus thuringiensis HD-789

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag BTF1_RS26950 Protein ID WP_000635965.1
Coordinates 5274412..5274762 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BTF1_RS26945 Protein ID WP_000004570.1
Coordinates 5274120..5274407 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTF1_RS26920 5269437..5270399 + 963 WP_000961146.1 UV DNA damage repair endonuclease UvsE -
BTF1_RS26925 5270392..5270964 - 573 WP_000908528.1 rhomboid family intramembrane serine protease -
BTF1_RS26930 5271058..5271417 + 360 WP_000583420.1 holo-ACP synthase -
BTF1_RS26935 5271574..5272524 + 951 WP_003309006.1 outer membrane lipoprotein carrier protein LolA -
BTF1_RS26940 5272642..5273811 + 1170 WP_000390594.1 alanine racemase -
BTF1_RS26945 5274120..5274407 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BTF1_RS26950 5274412..5274762 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BTF1_RS26955 5274830..5276998 + 2169 WP_000426211.1 RNA-binding transcriptional accessory protein -
BTF1_RS34350 5277056..5277172 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
BTF1_RS26960 5277368..5277826 + 459 WP_000343552.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T28171 WP_000635965.1 NC_018508:5274412-5274762 [Bacillus thuringiensis HD-789]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T28171 NC_018508:5274412-5274762 [Bacillus thuringiensis HD-789]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT28171 WP_000004570.1 NC_018508:5274120-5274407 [Bacillus thuringiensis HD-789]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT28171 NC_018508:5274120-5274407 [Bacillus thuringiensis HD-789]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References