Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 3897323..3897965 Replicon chromosome
Accession NC_018500
Organism Bacillus thuringiensis HD-771

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag BTG_RS19325 Protein ID WP_000635965.1
Coordinates 3897323..3897673 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BTG_RS19330 Protein ID WP_000004570.1
Coordinates 3897678..3897965 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTG_RS19315 3894259..3894717 - 459 WP_000343553.1 SprT family protein -
BTG_RS34370 3894913..3895029 + 117 WP_001143640.1 cortex morphogenetic protein CmpA -
BTG_RS19320 3895087..3897255 - 2169 WP_000426208.1 RNA-binding transcriptional accessory protein -
BTG_RS19325 3897323..3897673 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BTG_RS19330 3897678..3897965 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BTG_RS19335 3898278..3899447 - 1170 WP_000390595.1 alanine racemase -
BTG_RS19340 3899565..3900515 - 951 WP_003276258.1 outer membrane lipoprotein carrier protein LolA -
BTG_RS19345 3900672..3901031 - 360 WP_000583418.1 holo-ACP synthase -
BTG_RS19350 3901125..3901697 + 573 WP_000908529.1 rhomboid family intramembrane serine protease -
BTG_RS19355 3901690..3902652 - 963 WP_000961146.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T28169 WP_000635965.1 NC_018500:c3897673-3897323 [Bacillus thuringiensis HD-771]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T28169 NC_018500:c3897673-3897323 [Bacillus thuringiensis HD-771]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT28169 WP_000004570.1 NC_018500:c3897965-3897678 [Bacillus thuringiensis HD-771]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT28169 NC_018500:c3897965-3897678 [Bacillus thuringiensis HD-771]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References