Detailed information of TA system TA028168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 1530196..1531174
• Replicon: chromosome
• Accession: NC_018500
• Organism: Bacillus thuringiensis HD-771

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: BTG_RS07530
• Protein ID: WP_000624980.1
• Coordinates: 1530437..1531174 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: BTG_RS07525
• Protein ID: WP_000237818.1
• Coordinates: 1530196..1530324 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTG_RS07495	1525712..1525963	+	252	WP_000821942.1	response regulator transcription factor	-
BTG_RS07500	1526104..1526709	-	606	WP_000527413.1	hypothetical protein	-
BTG_RS07505	1526735..1527544	-	810	WP_000678376.1	C39 family peptidase	-
BTG_RS07510	1527857..1529326	-	1470	WP_000287537.1	beta-Ala-His dipeptidase	-
BTG_RS07515	1529538..1529927	+	390	WP_000712656.1	YxeA family protein	-
BTG_RS07520	1529947..1530123	-	177	WP_000852629.1	stage II sporulation protein SB	-
BTG_RS07525	1530196..1530324	-	129	WP_000237818.1	hypothetical protein	Antitoxin
BTG_RS07530	1530437..1531174	-	738	WP_000624980.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BTG_RS07535	1531413..1532150	-	738	WP_000594153.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
BTG_RS07540	1532317..1532799	-	483	WP_000191889.1	MarR family winged helix-turn-helix transcriptional regulator	-
BTG_RS07545	1532907..1533898	+	992	Protein_1516	hypothetical protein	-
BTG_RS07550	1534053..1534835	-	783	WP_000381780.1	class I SAM-dependent methyltransferase	-
BTG_RS07555	1535172..1535579	-	408	WP_000072495.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28369.10 Da
• Isoelectric Point: 8.2998

>T28168 WP_000624980.1 NC_018500:c1531174-1530437 [Bacillus thuringiensis HD-771]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNVFFDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T28168 NC_018500:c1531174-1530437 [Bacillus thuringiensis HD-771]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTATACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCTGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACCACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAAGAATATACAAAGAGCTTAAATGTATTTTTTGATTCATATGGGGAAAAAATTGGCGCGAAAATT
GTTGTTTTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCAGTGCAAGTAGAAAATGTCGGGATCATACCAGCAAAAATAGTAAGTGATG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT28168 WP_000237818.1 NC_018500:c1530324-1530196 [Bacillus thuringiensis HD-771]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT28168 NC_018500:c1530324-1530196 [Bacillus thuringiensis HD-771]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: W8YZL5