Detailed information of TA system TA028167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 4400663..4401636
• Replicon: chromosome
• Accession: NC_018491
• Organism: Bacillus cereus FRI-35

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: BCK_RS23130
• Protein ID: WP_014893739.1
• Coordinates: 4400899..4401636 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A151UPU1
• Locus tag: BCK_RS23125
• Protein ID: WP_000588714.1
• Coordinates: 4400663..4400791 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCK_RS23105	4397513..4398119	-	607	Protein_4418	CatB-related O-acetyltransferase	-
BCK_RS23110	4398320..4399789	-	1470	WP_000287562.1	beta-Ala-His dipeptidase	-
BCK_RS23115	4400006..4400395	+	390	WP_000714179.1	YxeA family protein	-
BCK_RS29755	4400414..4400590	-	177	WP_000852590.1	hypothetical protein	-
BCK_RS23125	4400663..4400791	-	129	WP_000588714.1	hypothetical protein	Antitoxin
BCK_RS23130	4400899..4401636	-	738	WP_014893739.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BCK_RS23135	4401974..4402711	-	738	WP_000594137.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
BCK_RS29085	4403048..4403212	-	165	Protein_4425	transposase	-
BCK_RS23140	4403320..4403802	-	483	WP_000191908.1	MarR family winged helix-turn-helix transcriptional regulator	-
BCK_RS23145	4403910..4405595	+	1686	WP_041184528.1	alpha-keto acid decarboxylase family protein	-
BCK_RS23150	4405773..4406555	-	783	WP_000381776.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28351.07 Da
• Isoelectric Point: 8.2998

>T28167 WP_014893739.1 NC_018491:c4401636-4400899 [Bacillus cereus FRI-35]
VISNIRIGLFILAIVFLVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMVKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNVFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPFEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T28167 NC_018491:c4401636-4400899 [Bacillus cereus FRI-35]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTGTATTTCA
TGGTAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCAAAATTTAGCGATATCGGCAAGACTGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATTATTCAAAATGTCAATCCGGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAAGAATATACAAAAAGCTTAAATGTATTTTTAGATTCGTATGGTGAAAAGATTGGGGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGCGGTATCCGTTCTCAATTTAGTATCATCGTTCCGTTTGA
ACATATTGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGTAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAGTAG

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5024.48 Da
• Isoelectric Point: 4.0932

>AT28167 WP_000588714.1 NC_018491:c4400791-4400663 [Bacillus cereus FRI-35]
VIKDYDNDFFKKDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT28167 NC_018491:c4400791-4400663 [Bacillus cereus FRI-35]
GTGATAAAAGATTATGACAATGATTTTTTTAAAAAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAGCTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: A0A151UPU1