Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1336542..1337184 Replicon chromosome
Accession NC_018491
Organism Bacillus cereus FRI-35

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BCK_RS06990 Protein ID WP_000635963.1
Coordinates 1336542..1336892 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BCK_RS06995 Protein ID WP_000004570.1
Coordinates 1336897..1337184 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCK_RS06975 1333478..1333936 - 459 WP_000344243.1 SprT family protein -
BCK_RS06980 1334132..1334248 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BCK_RS06985 1334306..1336474 - 2169 WP_014893363.1 RNA-binding transcriptional accessory protein -
BCK_RS06990 1336542..1336892 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BCK_RS06995 1336897..1337184 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BCK_RS07000 1337484..1338653 - 1170 WP_000390598.1 alanine racemase -
BCK_RS07005 1338772..1339722 - 951 WP_025991724.1 outer membrane lipoprotein carrier protein LolA -
BCK_RS07010 1339879..1340238 - 360 WP_000635038.1 holo-ACP synthase -
BCK_RS07015 1340331..1340903 + 573 WP_000906918.1 rhomboid family intramembrane serine protease -
BCK_RS07020 1340896..1341858 - 963 WP_000961147.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T28166 WP_000635963.1 NC_018491:c1336892-1336542 [Bacillus cereus FRI-35]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T28166 NC_018491:c1336892-1336542 [Bacillus cereus FRI-35]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT28166 WP_000004570.1 NC_018491:c1337184-1336897 [Bacillus cereus FRI-35]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT28166 NC_018491:c1337184-1336897 [Bacillus cereus FRI-35]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References