Detailed information of TA system TA028165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 3500236..3500741
• Replicon: chromosome
• Accession: NC_018485
• Organism: Methylocystis sp. SC2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: J7Q5C2
• Locus tag: BN69_RS16945
• Protein ID: WP_014892875.1
• Coordinates: 3500487..3500741 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relE
• Uniprot ID: J7QTA9
• Locus tag: BN69_RS16940
• Protein ID: WP_014892874.1
• Coordinates: 3500236..3500490 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN69_RS19515	3496938..3497135	-	198	WP_158491327.1	hypothetical protein	-
BN69_RS19520	3497212..3497517	-	306	WP_158491328.1	hypothetical protein	-
BN69_RS16930	3497582..3498175	+	594	WP_014892872.1	hypothetical protein	-
BN69_RS16935	3498286..3500091	+	1806	WP_014892873.1	translation elongation factor 4	-
BN69_RS16940	3500236..3500490	+	255	WP_014892874.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
BN69_RS16945	3500487..3500741	+	255	WP_014892875.1	Txe/YoeB family addiction module toxin	Toxin
BN69_RS16950	3500746..3501648	-	903	WP_014892876.1	DMT family transporter	-
BN69_RS16955	3501827..3502558	-	732	WP_014892877.1	class I SAM-dependent methyltransferase	-
BN69_RS16960	3502676..3503977	+	1302	WP_014892878.1	glutamate-5-semialdehyde dehydrogenase	-
BN69_RS16965	3504078..3504644	+	567	WP_148277302.1	nicotinate-nucleotide adenylyltransferase	-
BN69_RS16970	3504712..3505032	-	321	WP_014892880.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10141.63 Da
• Isoelectric Point: 9.0358

>T28165 WP_014892875.1 NC_018485:3500487-3500741 [Methylocystis sp. SC2]
MKLVFSEQAWADYLYWQGQDKKTLERINTLIKECARTPFEGLGKPEPLRGDLRGWWSRRIDREHRLVYRVEDGALLIAQC
RYHY

Nucleotide

• Length: 255 bp

>T28165 NC_018485:3500487-3500741 [Methylocystis sp. SC2]
ATGAAGCTCGTCTTCTCCGAACAGGCTTGGGCCGATTATCTCTATTGGCAGGGCCAAGATAAAAAGACGCTCGAACGCAT
CAATACGCTCATCAAAGAATGCGCCCGTACGCCCTTTGAGGGCCTTGGCAAGCCGGAACCCTTACGTGGCGATTTACGCG
GCTGGTGGTCGCGCCGCATCGATCGCGAGCATCGCCTCGTTTATCGCGTCGAAGACGGCGCCTTGCTGATTGCTCAGTGC
CGCTACCATTACTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9490.63 Da
• Isoelectric Point: 5.1651

>AT28165 WP_014892874.1 NC_018485:3500236-3500490 [Methylocystis sp. SC2]
MRTTSYSDLRKNLAATLDRVTEDHEPVVITRDKGKPAAVLISLEDFASYEETSYLLQSPRNAERLRAAIQELDAGRGNER
KLVE

Nucleotide

• Length: 255 bp

>AT28165 NC_018485:3500236-3500490 [Methylocystis sp. SC2]
ATGCGCACAACCTCTTACAGCGATCTCCGCAAGAATCTCGCCGCCACGCTCGATCGCGTCACGGAAGACCATGAGCCGGT
CGTCATCACCCGCGACAAAGGCAAGCCGGCTGCCGTGCTGATCTCGCTCGAAGACTTCGCCTCCTATGAAGAGACGAGCT
ATCTGCTGCAGAGTCCGCGCAATGCAGAGCGCTTGCGCGCCGCCATCCAGGAGCTCGACGCGGGGCGTGGAAATGAGCGC
AAGCTCGTTGAATGA

Structures

Toxin

• AlphaFold DB: J7Q5C2

Antitoxin

• AlphaFold DB: J7QTA9