Detailed information of TA system TA028164

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2616698..2617338
• Replicon: chromosome
• Accession: NC_018485
• Organism: Methylocystis sp. SC2

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: J7Q9J2
• Locus tag: BN69_RS12605
• Protein ID: WP_014892005.1
• Coordinates: 2617006..2617338 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: J7QHW2
• Locus tag: BN69_RS12600
• Protein ID: WP_014892004.1
• Coordinates: 2616698..2616955 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN69_RS12580	2612194..2613159	+	966	WP_014891999.1	preprotein translocase subunit SecG	-
BN69_RS12585	2613276..2614904	+	1629	WP_014892000.1	CTP synthase	-
BN69_RS12590	2615010..2615963	+	954	WP_014892001.1	hypothetical protein	-
BN69_RS12595	2616252..2616542	-	291	WP_014892003.1	GIY-YIG nuclease family protein	-
BN69_RS12600	2616698..2616955	+	258	WP_014892004.1	antitoxin	Antitoxin
BN69_RS12605	2617006..2617338	+	333	WP_014892005.1	type II toxin-antitoxin system ChpB family toxin	Toxin
BN69_RS12610	2617675..2618073	-	399	WP_014892006.1	carboxymuconolactone decarboxylase family protein	-
BN69_RS12620	2618572..2618871	+	300	WP_014892008.1	hypothetical protein	-
BN69_RS12625	2619090..2619539	+	450	WP_014892009.1	host attachment family protein	-
BN69_RS12630	2619782..2620999	+	1218	WP_014892010.1	alpha/beta hydrolase	-
BN69_RS12635	2621136..2622032	+	897	WP_014892011.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin PemI-like (5-83)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11589.34 Da
• Isoelectric Point: 8.0219

>T28164 WP_014892005.1 NC_018485:2617006-2617338 [Methylocystis sp. SC2]
MERGDIYLVSLDPTSGHEQQGARPVLVISPGAFNRLTKTPIVLPITSGGNFARTAGFAVSLIGAGTQTTGVIRCDQPRAL
DLGARRARRLEAAPPEIVEEALAKLAAILE

Nucleotide

• Length: 333 bp

>T28164 NC_018485:2617006-2617338 [Methylocystis sp. SC2]
ATGGAGCGCGGCGACATTTATCTCGTTTCGCTGGATCCGACCTCGGGGCACGAACAACAGGGCGCCCGGCCGGTGCTCGT
CATCTCGCCCGGCGCCTTCAACCGGCTGACGAAAACGCCGATCGTTCTGCCGATCACGAGCGGCGGCAATTTTGCGCGCA
CCGCAGGTTTCGCCGTTTCGCTGATCGGCGCCGGGACGCAGACGACCGGCGTCATCCGTTGCGACCAGCCGCGCGCGCTC
GATCTTGGCGCGCGCCGCGCCCGCCGGCTCGAAGCCGCGCCGCCAGAAATCGTCGAAGAGGCCTTGGCGAAACTCGCGGC
GATATTGGAATAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9138.40 Da
• Isoelectric Point: 4.3855

>AT28164 WP_014892004.1 NC_018485:2616698-2616955 [Methylocystis sp. SC2]
MHTTSLRKVGGSVMLAVPPAILDLLNLRAGATVGVSVDNGRLIIEPAARPRYTLEELLAQCDASAEPSEEDRAWLDDRPQ
GSELI

Nucleotide

• Length: 258 bp

>AT28164 NC_018485:2616698-2616955 [Methylocystis sp. SC2]
ATGCACACCACCTCTCTGCGCAAAGTCGGCGGCTCGGTCATGCTGGCGGTGCCGCCGGCGATTCTCGACCTGCTCAATCT
TCGGGCCGGGGCGACGGTGGGCGTCTCGGTGGACAACGGCCGGCTGATCATCGAGCCGGCGGCCAGGCCCCGCTACACGC
TCGAGGAGCTTCTGGCGCAATGCGACGCCTCGGCCGAGCCGTCTGAAGAAGACCGCGCCTGGCTCGACGACAGGCCGCAA
GGGTCAGAGCTGATTTAG

Structures

Toxin

• AlphaFold DB: J7Q9J2

Antitoxin

• AlphaFold DB: J7QHW2