Detailed information of TA system TA028163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1667543..1668150
• Replicon: chromosome
• Accession: NC_018485
• Organism: Methylocystis sp. SC2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: J7Q496
• Locus tag: BN69_RS08045
• Protein ID: WP_014891088.1
• Coordinates: 1667543..1667731 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BN69_RS08050
• Protein ID: WP_014891089.1
• Coordinates: 1667734..1668150 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN69_RS07995	1662779..1663198	-	420	WP_041926863.1	hypothetical protein	-
BN69_RS08000	1663195..1663416	-	222	WP_014891079.1	hypothetical protein	-
BN69_RS08005	1663416..1663733	-	318	WP_014891080.1	hypothetical protein	-
BN69_RS08010	1663754..1663996	-	243	WP_014891081.1	hypothetical protein	-
BN69_RS08015	1664009..1664239	-	231	WP_014891082.1	hypothetical protein	-
BN69_RS08020	1664474..1664746	-	273	WP_014891083.1	hypothetical protein	-
BN69_RS08025	1664743..1665138	-	396	WP_014891084.1	HNH endonuclease	-
BN69_RS18415	1665138..1666034	-	897	WP_014891085.1	phage related protein, Gp53	-
BN69_RS19435	1666173..1666340	-	168	WP_158491302.1	hypothetical protein	-
BN69_RS08035	1666485..1667030	+	546	WP_148277067.1	hypothetical protein	-
BN69_RS08040	1667150..1667434	+	285	WP_014891087.1	hypothetical protein	-
BN69_RS08045	1667543..1667731	+	189	WP_014891088.1	type II toxin-antitoxin system HicA family toxin	Toxin
BN69_RS08050	1667734..1668150	+	417	WP_014891089.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BN69_RS08055	1668164..1668784	-	621	WP_014891090.1	hypothetical protein	-
BN69_RS08060	1668837..1669730	-	894	WP_014891091.1	BRCT domain-containing protein	-
BN69_RS18420	1669754..1670530	-	777	WP_083858706.1	S24 family peptidase	-
BN69_RS08075	1670874..1671056	+	183	WP_148277068.1	hypothetical protein	-
BN69_RS08080	1671258..1671527	-	270	WP_014891095.1	hypothetical protein	-
BN69_RS19440	1671699..1671842	+	144	WP_158491303.1	hypothetical protein	-
BN69_RS19130	1672570..1672905	+	336	WP_148277069.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1652100..1712747	60647

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6839.97 Da
• Isoelectric Point: 11.0457

>T28163 WP_014891088.1 NC_018485:1667543-1667731 [Methylocystis sp. SC2]
MNSRQAKRYLAQKGATFYPGKGGHLHVTLNGKRSVLPQHGGSKDIPKGLWLSILKQLGIEEK

Nucleotide

• Length: 189 bp

>T28163 NC_018485:1667543-1667731 [Methylocystis sp. SC2]
ATGAACAGTAGGCAGGCCAAGCGCTATCTCGCCCAAAAGGGAGCGACGTTCTATCCCGGCAAAGGCGGACACCTTCACGT
CACGCTTAACGGCAAGCGCTCGGTCCTACCGCAGCATGGCGGATCGAAAGACATTCCCAAGGGGCTCTGGCTTTCGATCC
TCAAGCAGCTCGGCATAGAGGAAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14635.95 Da
• Isoelectric Point: 6.4713

>AT28163 WP_014891089.1 NC_018485:1667734-1668150 [Methylocystis sp. SC2]
MRYAVTLTKDGDGISVCVPDILEALTFGEDRDDALARASDAIETALMGYMAAREDIPAPKAKGRHYVTLPALSAAKIELY
SAMRAAGLGKAALAKRLGVALPQIDRLLDLGHASRLDAIERAFAVLGKTISIEVRDAA

Nucleotide

• Length: 417 bp

>AT28163 NC_018485:1667734-1668150 [Methylocystis sp. SC2]
ATGCGTTACGCCGTCACGCTCACCAAAGACGGAGACGGGATTTCCGTTTGCGTCCCCGACATCCTGGAAGCCCTTACCTT
TGGTGAGGATCGGGACGATGCGCTCGCCCGCGCGTCTGATGCGATCGAAACCGCCCTCATGGGCTATATGGCCGCCCGTG
AAGACATTCCGGCGCCAAAGGCGAAAGGGCGTCACTACGTCACGCTGCCCGCCCTTTCCGCAGCGAAGATCGAACTCTAT
TCGGCGATGCGCGCCGCCGGACTCGGAAAGGCCGCGCTCGCCAAGCGGCTCGGCGTCGCCCTGCCGCAGATCGACCGATT
GCTTGATCTCGGCCATGCCTCGCGGCTCGACGCGATCGAGCGCGCCTTTGCCGTGCTCGGCAAGACCATCAGCATCGAAG
TGCGCGACGCCGCGTAG

Structures

Toxin

• AlphaFold DB: J7Q496