Detailed information of TA system TA028161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 26154..26710
• Replicon: chromosome
• Accession: NC_018485
• Organism: Methylocystis sp. SC2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: J7QKR2
• Locus tag: BN69_RS00140
• Protein ID: WP_014889512.1
• Coordinates: 26154..26516 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J7Q3B4
• Locus tag: BN69_RS00145
• Protein ID: WP_014889513.1
• Coordinates: 26513..26710 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN69_RS00120	22048..23394	+	1347	WP_041926722.1	3-phosphoshikimate 1-carboxyvinyltransferase	-
BN69_RS00125	23391..24017	+	627	WP_014889509.1	(d)CMP kinase	-
BN69_RS00130	24025..25143	-	1119	WP_014889510.1	helix-turn-helix transcriptional regulator	-
BN69_RS00135	25263..26159	+	897	WP_014889511.1	tRNA glutamyl-Q(34) synthetase GluQRS	-
BN69_RS00140	26154..26516	-	363	WP_014889512.1	PIN domain-containing protein	Toxin
BN69_RS00145	26513..26710	-	198	WP_014889513.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BN69_RS00150	26865..27131	+	267	WP_014889514.1	acyl carrier protein	-
BN69_RS00155	27182..29197	-	2016	WP_014889515.1	M23 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13097.38 Da
• Isoelectric Point: 6.4791

>T28161 WP_014889512.1 NC_018485:c26516-26154 [Methylocystis sp. SC2]
VILVDTSIWVDHFRQPNARLVALLNDAEVLTHGFVIGELALGNLANRKVILSLLSDLPRAPLADEKEILELIERRALHGV
GIGYVDAHLIAAALLACPAALWTRDKRLQSVAERIGIPVL

Nucleotide

• Length: 363 bp

>T28161 NC_018485:c26516-26154 [Methylocystis sp. SC2]
GTGATTCTGGTCGACACGTCCATATGGGTCGACCACTTTCGCCAACCGAACGCTCGGCTCGTCGCATTGCTAAACGACGC
TGAAGTATTGACGCATGGTTTCGTCATCGGCGAACTCGCGCTTGGCAATCTCGCGAACCGCAAGGTCATATTGTCGCTCT
TAAGCGACCTTCCGAGAGCGCCGCTCGCAGACGAGAAAGAAATCCTGGAGTTGATCGAACGGCGCGCGTTACATGGCGTG
GGGATCGGTTACGTTGACGCGCACCTTATCGCCGCGGCGCTACTGGCTTGCCCGGCGGCGCTCTGGACGCGGGACAAGCG
ACTGCAATCAGTCGCCGAACGCATTGGAATTCCCGTTCTATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7345.46 Da
• Isoelectric Point: 11.3253

>AT28161 WP_014889513.1 NC_018485:c26710-26513 [Methylocystis sp. SC2]
MRTTIALDDELVAKAQDFTGLTEKSALIREALKALIERESARRLARLGGSEPQLRDIPRRRSTPR

Nucleotide

• Length: 198 bp

>AT28161 NC_018485:c26710-26513 [Methylocystis sp. SC2]
ATGCGCACGACGATCGCGCTCGACGATGAGCTCGTCGCCAAGGCGCAGGATTTCACCGGACTTACGGAAAAGTCTGCGCT
AATCCGCGAAGCGTTAAAGGCGCTCATTGAGAGGGAAAGCGCTCGGAGATTAGCGCGTCTCGGCGGCTCCGAGCCACAGC
TCCGGGATATTCCTCGACGCCGGTCGACCCCGAGGTGA

Structures

Toxin

• AlphaFold DB: J7QKR2

Antitoxin

• AlphaFold DB: J7Q3B4