Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2847075..2847646 Replicon chromosome
Accession NZ_CP124894
Organism Enterococcus faecalis strain EfsC116

Toxin (Protein)


Gene name mazF Uniprot ID R3GRA7
Locus tag QLQ63_RS13885 Protein ID WP_002360937.1
Coordinates 2847075..2847416 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3JGB1
Locus tag QLQ63_RS13890 Protein ID WP_002354773.1
Coordinates 2847416..2847646 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QLQ63_RS13880 (2843090) 2843090..2846704 - 3615 WP_002389470.1 DNA-directed RNA polymerase subunit beta -
QLQ63_RS13885 (2847075) 2847075..2847416 - 342 WP_002360937.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
QLQ63_RS13890 (2847416) 2847416..2847646 - 231 WP_002354773.1 hypothetical protein Antitoxin
QLQ63_RS13895 (2848053) 2848053..2848268 - 216 WP_002354768.1 zinc ribbon domain-containing protein -
QLQ63_RS13900 (2848407) 2848407..2849399 + 993 WP_023894581.1 biotin--[acetyl-CoA-carboxylase] ligase -
QLQ63_RS13905 (2849663) 2849663..2850301 - 639 WP_002378863.1 lytic polysaccharide monooxygenase -
QLQ63_RS13910 (2850987) 2850987..2852603 + 1617 WP_047648929.1 phosphatase PAP2/LCP family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13165.41 Da        Isoelectric Point: 9.3988

>T281561 WP_002360937.1 NZ_CP124894:c2847416-2847075 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.67 Da        Isoelectric Point: 4.3313

>AT281561 WP_002354773.1 NZ_CP124894:c2847646-2847416 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A7G9

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2A812

References