Detailed information of TA system TA028150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1764842..1765639
• Replicon: chromosome
• Accession: NC_018405
• Organism: Enterobacter cloacae subsp. cloacae ENHKU01

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: ECENHK_RS08325
• Protein ID: WP_173674980.1
• Coordinates: 1765115..1765639 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A2W0G3H3
• Locus tag: ECENHK_RS08320
• Protein ID: WP_014883303.1
• Coordinates: 1764842..1765111 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECENHK_RS08290	1760462..1760962	+	501	WP_014883299.1	DedA family protein	-
ECENHK_RS08295	1761066..1762308	+	1243	Protein_1614	bifunctional glucose-1-phosphatase/inositol phosphatase	-
ECENHK_RS08300	1762342..1762569	-	228	WP_014883300.1	YccJ family protein	-
ECENHK_RS08305	1762588..1763184	-	597	WP_014883301.1	NAD(P)H:quinone oxidoreductase	-
ECENHK_RS08310	1763576..1763746	+	171	WP_023332656.1	general stress protein	-
ECENHK_RS08315	1763863..1764780	+	918	WP_014883302.1	DMT family transporter	-
ECENHK_RS08320	1764842..1765111	+	270	WP_014883303.1	DUF1778 domain-containing protein	Antitoxin
ECENHK_RS08325	1765115..1765639	+	525	WP_173674980.1	GNAT family N-acetyltransferase	Toxin
ECENHK_RS08330	1765713..1767035	-	1323	WP_014883305.1	pyrimidine utilization transport protein G	-
ECENHK_RS08335	1767057..1767551	-	495	WP_014883306.1	pyrimidine utilization flavin reductase protein F	-
ECENHK_RS08340	1767561..1768151	-	591	WP_014883307.1	malonic semialdehyde reductase	-
ECENHK_RS08345	1768161..1768961	-	801	WP_014883308.1	pyrimidine utilization protein D	-
ECENHK_RS08350	1768969..1769355	-	387	WP_014883309.1	pyrimidine utilization protein C	-
ECENHK_RS08355	1769367..1770056	-	690	WP_014883310.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (90-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19662.52 Da
• Isoelectric Point: 8.4738

>T28150 WP_173674980.1 NC_018405:1765115-1765639 [Enterobacter kobei]
IVANLTIEMFSEGSDYDFRGFDCGEPSLNAFLTEHLVRQHKGRILRGYLLKDRDHPRVLGYYTLSGSCFEKAMLPSTTQQ
RRIPYVNVPSVTLGRLAVHKDLQGKEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNEQAKRFYLKQGFIPLTGENSHSL
FFPTKSIEQLFEQA

Nucleotide

• Length: 525 bp

>T28150 NC_018405:1765115-1765639 [Enterobacter kobei]
ATCGTGGCAAATCTCACGATCGAAATGTTTTCAGAGGGAAGTGATTACGATTTCAGGGGCTTCGATTGTGGTGAACCCTC
GCTAAATGCCTTCCTGACGGAACATCTGGTGCGTCAGCACAAGGGACGGATATTGCGAGGTTACCTGCTTAAAGATCGTG
ACCATCCGCGTGTGCTGGGCTATTACACGTTGTCCGGAAGCTGCTTTGAAAAAGCGATGCTTCCTTCTACGACCCAGCAG
CGTCGGATCCCCTATGTCAACGTACCCAGTGTGACGCTGGGGCGTCTGGCTGTGCATAAAGATCTACAGGGAAAGGAGTG
GGGAACAACGCTCGTCGCACATGCTATGCGCGTTGTGTATCTGGCATCGCAGGCGGTAGGGGTACACGGAATCTTTGTTG
ATGCTCTCAATGAACAGGCAAAACGATTCTATCTGAAGCAGGGTTTTATTCCGCTGACAGGTGAAAACAGCCATTCTCTT
TTCTTCCCCACAAAATCTATCGAACAACTCTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10278.72 Da
• Isoelectric Point: 5.8836

>AT28150 WP_014883303.1 NC_018405:1764842-1765111 [Enterobacter kobei]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNESSWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT28150 NC_018405:1764842-1765111 [Enterobacter kobei]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAGACAATCACCCAGTTTATGGTGAACAGTGCGTCAGAACGCGCTGCTGAAGTCATCGAACAACATCGGCGTC
TCGTATTAAACGAGTCATCATGGAATGCGGTGATGGACGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCAAAACGTCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2W0G3H3