Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1154470..1155090 Replicon chromosome
Accession NC_018405
Organism Enterobacter cloacae subsp. cloacae ENHKU01

Toxin (Protein)


Gene name Hha Uniprot ID A0A2W0G488
Locus tag ECENHK_RS05455 Protein ID WP_014882802.1
Coordinates 1154470..1154688 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag ECENHK_RS05460 Protein ID WP_008499288.1
Coordinates 1154716..1155090 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECENHK_RS05425 1150479..1150739 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
ECENHK_RS05430 1150742..1150882 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
ECENHK_RS05435 1150879..1151589 - 711 WP_014882798.1 GNAT family N-acetyltransferase -
ECENHK_RS05440 1151691..1153151 + 1461 WP_014882799.1 PLP-dependent aminotransferase family protein -
ECENHK_RS05445 1153123..1153590 - 468 WP_014882800.1 membrane protein -
ECENHK_RS05450 1153709..1154260 - 552 WP_014882801.1 maltose O-acetyltransferase -
ECENHK_RS05455 1154470..1154688 - 219 WP_014882802.1 hemolysin expression modulator Hha Toxin
ECENHK_RS05460 1154716..1155090 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
ECENHK_RS05465 1155602..1158748 - 3147 WP_014882803.1 multidrug efflux RND transporter permease subunit -
ECENHK_RS05470 1158771..1159964 - 1194 WP_014882804.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T28148 WP_014882802.1 NC_018405:c1154688-1154470 [Enterobacter kobei]
MSEKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T28148 NC_018405:c1154688-1154470 [Enterobacter kobei]
ATGTCTGAAAAACCACTCACAAAGGTCGATTATTTGATGCGCTTACGCCGTTGTCAGTCAATTGACACCCTCGAACGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCTGACCATCGCCTCGCTGAGC
TAACGATGAATAAGTTATATGACAAAATTCCCACATCCGTGTGGAAATTTGTACGTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT28148 WP_008499288.1 NC_018405:c1155090-1154716 [Enterobacter kobei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT28148 NC_018405:c1155090-1154716 [Enterobacter kobei]
ATGGACGAGTACTCGCCAAAACGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGATCCAACGTCTGCCATCAATTTACAGTTGAACGAGCTTATAGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCGCAAGATTTGCAAAAATGGCGCAAATCGGGAAACCGACT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCCGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G488

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References