Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5143060..5143685 Replicon chromosome
Accession NZ_CP124873
Organism Klebsiella pneumoniae strain K82

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag QKW38_RS25075 Protein ID WP_019705794.1
Coordinates 5143060..5143443 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag QKW38_RS25080 Protein ID WP_004150355.1
Coordinates 5143443..5143685 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QKW38_RS25060 (5140426) 5140426..5141328 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
QKW38_RS25065 (5141325) 5141325..5141960 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
QKW38_RS25070 (5141957) 5141957..5142886 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
QKW38_RS25075 (5143060) 5143060..5143443 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
QKW38_RS25080 (5143443) 5143443..5143685 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
QKW38_RS25085 (5143890) 5143890..5144807 + 918 WP_004146235.1 alpha/beta hydrolase -
QKW38_RS25090 (5144822) 5144822..5145763 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
QKW38_RS25095 (5145808) 5145808..5146245 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
QKW38_RS25100 (5146242) 5146242..5147102 - 861 WP_004146232.1 virulence factor BrkB family protein -
QKW38_RS25105 (5147096) 5147096..5147695 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T281381 WP_019705794.1 NZ_CP124873:c5143443-5143060 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT281381 WP_004150355.1 NZ_CP124873:c5143685-5143443 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References