Detailed information of TA system TA028138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 8326..9409
• Replicon: plasmid EFD32pA
• Accession: NC_018222
• Organism: Enterococcus faecalis D32

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: EFD32_RS16160
• Protein ID: WP_014862453.1
• Coordinates: 8600..9409 (+)
• Length: 270 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL01
• Locus tag: EFD32_RS15040
• Protein ID: WP_002331065.1
• Coordinates: 8326..8598 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFD32_RS15015	5336..5577	+	242	Protein_5	peptide-binding protein	-
EFD32_RS15020	5626..5709	+	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
EFD32_RS15025	5834..6571	+	738	WP_002292226.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
EFD32_RS16155	6741..7001	+	261	Protein_8	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
EFD32_RS15030	7104..8000	+	897	WP_014862451.1	ParA family protein	-
EFD32_RS15035	8099..8308	+	210	WP_000527318.1	peptide-binding protein	-
EFD32_RS15040	8326..8598	+	273	WP_002331065.1	antitoxin	Antitoxin
EFD32_RS16160	8600..9409	+	810	WP_014862453.1	zeta toxin family protein	Toxin
EFD32_RS15050	9443..10123	+	681	WP_000191454.1	IS6-like element ISEnfa1 family transposase	-
EFD32_RS16460	10178..10267	-	90	Protein_14	aminoglycoside 6-adenylyltransferase	-
EFD32_RS15060	10872..11538	+	667	Protein_15	ABC transporter ATP-binding protein	-
EFD32_RS16465	11723..11821	-	99	WP_142435003.1	type I toxin-antitoxin system Fst family toxin	-
EFD32_RS15065	12244..12492	+	249	WP_014862456.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B)	-	1..12893	12893
	-	inside	IS/Tn	erm(B)	-	5834..10123	4289

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 270 a.a.
• Molecular weight: 30634.89 Da
• Isoelectric Point: 6.9747

>T28138 WP_014862453.1 NC_018222:8600-9409 [Enterococcus faecalis D32]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQGFCCKVSDKLILR

Nucleotide

• Length: 810 bp

>T28138 NC_018222:8600-9409 [Enterococcus faecalis D32]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCGAATCGCATGACAGAAGCACTTATTAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGAACAGGACGAACAACAGACGTTCCCATTCAAACAGCCACAATGCTTCAAT
CTAAAGGTTATGAAACAAAAATATACGTCATGGCAGTACCGAAAATTGAATCATACTTAGGAACGATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCTAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGGGTTCTGTTGCAAAGTTTCCGATAAGCTCAT
TTTGAGGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10733.17 Da
• Isoelectric Point: 4.4865

>AT28138 WP_002331065.1 NC_018222:8326-8598 [Enterococcus faecalis D32]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Nucleotide

• Length: 273 bp

>AT28138 NC_018222:8326-8598 [Enterococcus faecalis D32]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A828ZL55