Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4526543..4527353 Replicon chromosome
Accession NZ_CP124873
Organism Klebsiella pneumoniae strain K82

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag QKW38_RS22205 Protein ID WP_004178461.1
Coordinates 4526543..4527076 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag QKW38_RS22210 Protein ID WP_002887278.1
Coordinates 4527087..4527353 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QKW38_RS22200 (4525374) 4525374..4526495 + 1122 WP_004186850.1 cupin domain-containing protein -
QKW38_RS22205 (4526543) 4526543..4527076 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
QKW38_RS22210 (4527087) 4527087..4527353 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
QKW38_RS22215 (4527456) 4527456..4528889 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
QKW38_RS22220 (4528879) 4528879..4529562 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
QKW38_RS22225 (4529735) 4529735..4531120 + 1386 WP_064152126.1 efflux transporter outer membrane subunit -
QKW38_RS22230 (4531138) 4531138..4531482 + 345 WP_064152125.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T281378 WP_004178461.1 NZ_CP124873:c4527076-4526543 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT281378 WP_002887278.1 NZ_CP124873:c4527353-4527087 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References