Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 55891..56534 Replicon plasmid pK6328_1
Accession NZ_CP124838
Organism Klebsiella pneumoniae strain KPN6328

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag QHR28_RS27335 Protein ID WP_001044770.1
Coordinates 55891..56307 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag QHR28_RS27340 Protein ID WP_001261282.1
Coordinates 56304..56534 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QHR28_RS27315 (51994) 51994..52266 - 273 Protein_61 transposase -
QHR28_RS27325 (53248) 53248..54270 - 1023 WP_000361404.1 helicase UvrD -
QHR28_RS27330 (54255) 54255..55817 - 1563 WP_004206609.1 AAA family ATPase -
QHR28_RS27335 (55891) 55891..56307 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
QHR28_RS27340 (56304) 56304..56534 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QHR28_RS27345 (56491) 56491..56952 + 462 WP_014343465.1 hypothetical protein -
QHR28_RS27350 (57113) 57113..58057 + 945 WP_011977810.1 hypothetical protein -
QHR28_RS27355 (58094) 58094..58486 + 393 WP_011977811.1 hypothetical protein -
QHR28_RS27360 (58544) 58544..59065 + 522 WP_013214008.1 hypothetical protein -
QHR28_RS27365 (59111) 59111..59314 + 204 WP_011977813.1 hypothetical protein -
QHR28_RS27370 (59344) 59344..60348 + 1005 WP_011977814.1 hypothetical protein -
QHR28_RS27375 (60532) 60532..61311 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-33 / blaTEM-1B / rmtB - 1..119030 119030


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T281344 WP_001044770.1 NZ_CP124838:c56307-55891 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT281344 WP_001261282.1 NZ_CP124838:c56534-56304 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References