Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2868989..2869560 Replicon chromosome
Accession NC_018221
Organism Enterococcus faecalis D32

Toxin (Protein)


Gene name mazF Uniprot ID R3GRA7
Locus tag EFD32_RS14425 Protein ID WP_002360937.1
Coordinates 2868989..2869330 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3JGB1
Locus tag EFD32_RS14430 Protein ID WP_002354773.1
Coordinates 2869330..2869560 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EFD32_RS14420 2865005..2868619 - 3615 WP_002389470.1 DNA-directed RNA polymerase subunit beta -
EFD32_RS14425 2868989..2869330 - 342 WP_002360937.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EFD32_RS14430 2869330..2869560 - 231 WP_002354773.1 hypothetical protein Antitoxin
EFD32_RS14435 2869967..2870182 - 216 WP_002354768.1 zinc ribbon domain-containing protein -
EFD32_RS14440 2870321..2871313 + 993 WP_002358973.1 biotin--[acetyl-CoA-carboxylase] ligase -
EFD32_RS14445 2871577..2872206 - 630 WP_002388632.1 lytic polysaccharide monooxygenase -
EFD32_RS14455 2872899..2874515 + 1617 WP_002372620.1 phosphatase PAP2/LCP family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13165.41 Da        Isoelectric Point: 9.3988

>T28130 WP_002360937.1 NC_018221:c2869330-2868989 [Enterococcus faecalis D32]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T28130 NC_018221:c2869330-2868989 [Enterococcus faecalis D32]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGGCAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.67 Da        Isoelectric Point: 4.3313

>AT28130 WP_002354773.1 NC_018221:c2869560-2869330 [Enterococcus faecalis D32]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Download         Length: 231 bp

>AT28130 NC_018221:c2869560-2869330 [Enterococcus faecalis D32]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A7G9

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2A812

References