Detailed information of TA system TA028129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_26(antitoxin)
• Location: 2504068..2504763
• Replicon: chromosome
• Accession: NC_018221
• Organism: Enterococcus faecalis D32

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: E2YXR2
• Locus tag: EFD32_RS12690
• Protein ID: WP_002380839.1
• Coordinates: 2504419..2504763 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: EFD32_RS12685
• Protein ID: WP_002373919.1
• Coordinates: 2504068..2504400 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFD32_RS12635	2499830..2500645	-	816	WP_010730098.1	PD-(D/E)XK nuclease-like domain-containing protein	-
EFD32_RS12640	2500608..2501624	-	1017	WP_041161741.1	recombinase RecT	-
EFD32_RS12650	2501717..2502073	-	357	WP_010774955.1	hypothetical protein	-
EFD32_RS12655	2502076..2502300	-	225	WP_002367281.1	hypothetical protein	-
EFD32_RS12660	2502297..2502596	-	300	WP_014862416.1	hypothetical protein	-
EFD32_RS12665	2502683..2503099	-	417	WP_010730101.1	hypothetical protein	-
EFD32_RS12670	2503100..2503270	-	171	WP_002405829.1	hypothetical protein	-
EFD32_RS12675	2503305..2503574	-	270	WP_002367284.1	hypothetical protein	-
EFD32_RS12680	2503587..2503769	-	183	WP_002358110.1	hypothetical protein	-
EFD32_RS12685	2504068..2504400	+	333	WP_002373919.1	helix-turn-helix domain-containing protein	Antitoxin
EFD32_RS12690	2504419..2504763	+	345	WP_002380839.1	ImmA/IrrE family metallo-endopeptidase	Toxin
EFD32_RS12695	2504821..2505549	+	729	WP_002380840.1	potassium channel family protein	-
EFD32_RS12700	2505704..2506883	+	1180	Protein_2406	site-specific integrase	-
EFD32_RS12705	2506986..2507135	-	150	WP_002356321.1	50S ribosomal protein L33	-
EFD32_RS12710	2507261..2509399	-	2139	WP_002372359.1	penicillin-binding protein 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2465791..2506883	41092

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (21-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-59)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13640.59 Da
• Isoelectric Point: 5.2241

>T28129 WP_002380839.1 NC_018221:2504419-2504763 [Enterococcus faecalis D32]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPSEQEEAIYHELKHVKDHVDIMELYKIPAFRSKMESEAEQ
YMFRSLIEKYEGQYNYSNVIAHYNLKMGQEIYLK

Nucleotide

• Length: 345 bp

>T28129 NC_018221:2504419-2504763 [Enterococcus faecalis D32]
ATGAAAAGTATCAAAGAGTTGGTAGAAGAATATAATGTGGAGTTAGTTTTTACTACTTTGAACAAACGCGCATGTTTCGA
CCCTACCTACGGTATCATATTTGTAAATCAAAATTTAACACCATCAGAACAAGAAGAAGCAATATATCACGAGTTAAAGC
ATGTAAAAGACCATGTGGATATAATGGAATTGTATAAAATTCCTGCTTTTCGTTCTAAGATGGAATCCGAAGCAGAACAA
TATATGTTTAGAAGCTTAATCGAAAAATATGAAGGACAATACAATTATTCAAATGTTATAGCTCATTACAACTTAAAAAT
GGGACAAGAAATTTATTTGAAATAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12734.28 Da
• Isoelectric Point: 5.2423

>AT28129 WP_002373919.1 NC_018221:2504068-2504400 [Enterococcus faecalis D32]
MNTYEIIKELTKRKKMSIRQLEITLGYSNGYFSKWKKVSPNSEGLQKVADYFNVSVDYLLGRTDNTKATDEKNSDDLDDV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT28129 NC_018221:2504068-2504400 [Enterococcus faecalis D32]
ATGAATACTTATGAAATAATAAAAGAGTTGACAAAAAGGAAGAAAATGTCTATTCGACAATTAGAAATTACTCTAGGTTA
CTCAAATGGATATTTTAGCAAGTGGAAAAAAGTTTCTCCAAACTCAGAAGGCCTACAAAAAGTTGCGGACTACTTCAATG
TATCGGTAGATTATCTATTGGGAAGAACTGATAATACTAAAGCAACTGATGAAAAAAATTCTGATGATTTAGATGATGTA
CTGGATAACGTCATGAGTTTTGACGGTGAACCGCTTGATGATCATGACAGAGAAGTTATCCGTGCATATTTAAAGGGTAG
ATTCGGGAAATAA

Structures

Toxin

• AlphaFold DB: A0A0M2A9N7