Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4549911..4550494 Replicon chromosome
Accession NZ_CP124819
Organism Escherichia coli strain C325

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U9LIA0
Locus tag QLG17_RS22610 Protein ID WP_000254747.1
Coordinates 4550159..4550494 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag QLG17_RS22605 Protein ID WP_000581937.1
Coordinates 4549911..4550159 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QLG17_RS22595 (4546250) 4546250..4547551 + 1302 WP_000046821.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
QLG17_RS22600 (4547599) 4547599..4549833 + 2235 WP_000226815.1 GTP pyrophosphokinase -
QLG17_RS22605 (4549911) 4549911..4550159 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
QLG17_RS22610 (4550159) 4550159..4550494 + 336 WP_000254747.1 endoribonuclease MazF Toxin
QLG17_RS22615 (4550565) 4550565..4551356 + 792 WP_001071643.1 nucleoside triphosphate pyrophosphohydrolase -
QLG17_RS22620 (4551584) 4551584..4553221 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
QLG17_RS22625 (4553309) 4553309..4554607 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T281287 WP_000254747.1 NZ_CP124819:4550159-4550494 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLYVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT281287 WP_000581937.1 NZ_CP124819:4549911-4550159 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LIA0

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References