Detailed information of TA system TA028125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 1930828..1931347
• Replicon: chromosome
• Accession: NC_018221
• Organism: Enterococcus faecalis D32

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: S4D6W6
• Locus tag: EFD32_RS09660
• Protein ID: WP_016617334.1
• Coordinates: 1930828..1931085 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: S4BX41
• Locus tag: EFD32_RS09665
• Protein ID: WP_014862251.1
• Coordinates: 1931078..1931347 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFD32_RS09635	1925932..1926540	-	609	WP_014862246.1	hypothetical protein	-
EFD32_RS09640	1927073..1928616	-	1544	Protein_1813	hypothetical protein	-
EFD32_RS16505	1929271..1929411	+	141	WP_187287606.1	hypothetical protein	-
EFD32_RS16335	1929371..1929554	+	184	Protein_1815	hypothetical protein	-
EFD32_RS09655	1929916..1930446	-	531	WP_014862250.1	hypothetical protein	-
EFD32_RS09660	1930828..1931085	-	258	WP_016617334.1	Txe/YoeB family addiction module toxin	Toxin
EFD32_RS09665	1931078..1931347	-	270	WP_014862251.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EFD32_RS09670	1931892..1932428	+	537	WP_080019837.1	hypothetical protein	-
EFD32_RS09675	1932442..1932942	+	501	WP_014862253.1	hypothetical protein	-
EFD32_RS09680	1933486..1934052	+	567	WP_014862254.1	hypothetical protein	-
EFD32_RS09685	1934059..1934304	+	246	WP_016619114.1	SpoVT/AbrB-like protein	-
EFD32_RS09690	1934294..1934659	+	366	WP_014862256.1	type II toxin-antitoxin system PemK/MazF family toxin	-
EFD32_RS09695	1935233..1935682	+	450	WP_014862257.1	hypothetical protein	-
EFD32_RS09700	1935698..1936084	+	387	WP_014862258.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10192.51 Da
• Isoelectric Point: 8.4368

>T28125 WP_016617334.1 NC_018221:c1931085-1930828 [Enterococcus faecalis D32]
MIKAWSDDAWDDYLYWHDQGNKSNIKKINRLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYKVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T28125 NC_018221:c1931085-1930828 [Enterococcus faecalis D32]
ATGATTAAAGCTTGGTCTGATGATGCTTGGGATGACTATCTTTATTGGCATGATCAAGGGAACAAAAGCAACATAAAAAA
AATTAATAGGTTAATAAAAGATATAGATCGTTCGCCCTTTGCTGGATTAGGTAAACCCGAACCATTGAAGCATGATTTAT
CTGGGAAATGGTCTAGAAGAATTACAGATGAGCATAGGCTGATATATAAAGTTGAAAATGAAACGATATTCATTTATTCT
GCCAAAGATCATTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10429.62 Da
• Isoelectric Point: 4.4453

>AT28125 WP_014862251.1 NC_018221:c1931347-1931078 [Enterococcus faecalis D32]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKEVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGLFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT28125 NC_018221:c1931347-1931078 [Enterococcus faecalis D32]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAACCTACGTAGTTATATGAAACAAGTTAATGAAGACGCTGAAACACT
TATTGTAACAAGTAAAGAAGTAGAAGATACAGTAGTGGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACTTTGA
GAACACTTAGTAATAATTATGTTATGGAAAAAATTCGTCGAGGAGATGAACAGTTTTCCAAAGGTTTATTTAAGACACAT
GACTTAATTGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: S4D6W6

Antitoxin

• AlphaFold DB: S4BX41